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. 2016 Mar 9:7:529-31.
doi: 10.1016/j.dib.2016.02.083. eCollection 2016 Jun.

Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples

Alessandro Tanca  1 , Maria Filippa Addis  1 , Salvatore Pisanu  1 , Marcello Abbondio  1 , Daniela Pagnozzi  1 , Albino Eccher  2 , Guido Rindi  3 , Paolo Cossu-Rocca  4 , Sergio Uzzau  5 , Giuseppe Fanciulli  6
Affiliations

Atypical carcinoid and large cell neuroendocrine carcinoma of the lung: a proteomic dataset from formalin-fixed archival samples

Alessandro Tanca et al. Data Brief. .

Abstract

Here we present a dataset generated using formalin-fixed paraffin-embedded archival samples from two rare lung neuroendocrine tumor subtypes (namely, two atypical carcinoids, ACs, and two large-cell neuroendocrine carcinomas, LCNECs). Samples were subjected to a shotgun proteomics pipeline, comprising full-length protein extraction, SDS removal through spin columns, in solution trypsin digestion, long gradient liquid chromatography peptide separation and LTQ-Orbitrap mass spectrometry analysis. A total of 1260 and 2436 proteins were identified in the AC and LCNEC samples, respectively, with FDR <1%. MS data are available in the PeptideAtlas repository at http://www.peptideatlas.org/PASS/PASS00375.

Keywords: Archival tissues; FFPE; Mass spectrometry; Neuroendocrine tumors; Proteomics.

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References

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    1. Tanca A., Abbondio M., Pisanu S., Pagnozzi D., Uzzau S., Addis M.F. Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissue. Clin. Proteomics. 2014;11:28. - PMC - PubMed
    1. Tanca A., Biosa G., Pagnozzi D., Addis M.F., Uzzau S. Comparison of detergent-based sample preparation workflows for LTQ-Orbitrap analysis of the Escherichia coli proteome. Proteomics. 2013;13:2597–2607. - PubMed