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Comment
. 2016 Aug;13(4):374-5.
doi: 10.1089/zeb.2016.29004.mat. Epub 2016 Apr 8.

Drug-Inducible, Cell-Specific Manipulation of Intracellular Calcium in Zebrafish Through Mammalian TRPV1 Expression

Molly A Matty  1 , Rebecca W Beerman  1 , David M Tobin  1   2
Affiliations
Comment

Drug-Inducible, Cell-Specific Manipulation of Intracellular Calcium in Zebrafish Through Mammalian TRPV1 Expression

Molly A Matty et al. Zebrafish. 2016 Aug.
No abstract available

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Figures

<b>FIG. 1.</b>
FIG. 1.
Expression of rTRPV1 allows for conditional and reversible control of calcium levels in specific cell types. (A) Cartoon of the Gateway construct used to generate the rTRPV1 transgenic lines. rTRPV1, initially in a Gateway pME vector, can be combined with any cell-specific promoter and fluorophore. (B) Different capsaicin treatments lead to varied cellular outcomes (green indicates calcium). Pulsed capsaicin can reversibly alter intracellular calcium levels; gradient application of capsaicin can change subcellular calcium dynamics; prolonged capsaicin treatment at high doses can induce cell death, ablating specific cell populations. (C) Transgenic zebrafish larvae Tg(LysC:rTRPV1-tdTomato; LysC:GCaMP3). GCaMP3 is a genetically encoded calcium indicator that exhibits increased fluorescence when bound by calcium. Scale bar is 200 μm. Boxed region is enlarged in (D), showing increased levels of GCaMP signal upon capsaicin administration, but no change in basal red fluorescence. rTRPV1, rat TRPV1. Color images available online at www.liebertpub.com/zeb
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References

    1. Beerman RW, Matty MA, Au GG, Looger LL, Choudhury KR, Keller PJ, et al. . Direct in vivo manipulation and imaging of calcium transients in neutrophils identify a critical role for leading-edge calcium flux. Cell Rep 2015;13:1–11 - PMC - PubMed

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