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. 2016 Mar 30:7:384.
doi: 10.3389/fmicb.2016.00384. eCollection 2016.

Sub-MIC Tylosin Inhibits Streptococcus suis Biofilm Formation and Results in Differential Protein Expression

Shuai Wang  1 , Yanbei Yang  1 , Yulin Zhao  1 , Honghai Zhao  2 , Jingwen Bai  1 , Jianqing Chen  1 , Yonghui Zhou  1 , Chang Wang  1 , Yanhua Li  1
Affiliations

Sub-MIC Tylosin Inhibits Streptococcus suis Biofilm Formation and Results in Differential Protein Expression

Shuai Wang et al. Front Microbiol. .

Abstract

Streptococcus suis (S.suis) is an important zoonotic pathogen that causes severe diseases in humans and pigs. Biofilms of S. suis can induce persistent infections that are difficult to treat. In this study, the effect of tylosin on biofilm formation of S. suis was investigated. 1/2 minimal inhibitory concentration (MIC) and 1/4 MIC of tylosin were shown to inhibit S. suis biofilm formation in vitro. By using the iTRAQ strategy, we compared the protein expression profiles of S. suis grown with sub-MIC tylosin treatment and with no treatment. A total of 1501 proteins were identified by iTRAQ. Ninety-six differentially expressed proteins were identified (Ratio > ±1.5, p < 0.05). Several metabolism proteins (such as phosphoglycerate kinase) and surface proteins (such as ABC transporter proteins) were found to be involved in biofilm formation. Our results indicated that S. suis metabolic regulation, cell surface proteins, and virulence proteins appear to be of importance in biofilm growth with sub-MIC tylosin treatment. Thus, our data revealed the rough regulation of biofilm formation that may provide a foundation for future research into mechanisms and targets.

Keywords: S. suis; biofilms; iTRAQ; proteomics; tylosin.

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Figures

Figure 1
Figure 1
Effect of tylosin at different concentrations on S. suis ATCC 700794 biofilm formation. The data are expressed as the means ± standard deviations. Significant decrease (*p < 0.05) compared with control biofilm formation of S. suis in vitro.
Figure 2
Figure 2
(A) Biofilm formation of S. suis without tylosin. (B) Biofilm formation of S. suis with 1/4 MIC tylosin treatment.
Figure 3
Figure 3
Effect of 1/4 MIC of tylosin on S. suis ATCC 700794 biofilm formation by colony forming unit (CFU) enumeration. The data are expressed as the means ± standard deviations. Significant decrease (*p < 0.05) compared with control biofilm formation of S. suis in vitro.
Figure 4
Figure 4
Annotation of differentially regulated protein (ratio >1.5 or < 0.67) functions by Gene Ontology (GO).
See this image and copyright information in PMC

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