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. 1977 Oct;74(10):4370-4.
doi: 10.1073/pnas.74.10.4370.

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation

B M Paterson, B E Roberts, E L Kuff

Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation

B M Paterson et al. Proc Natl Acad Sci U S A. 1977 Oct.

Abstract

We present a simple method for directly correlating structural gene sequences in DNA with their corresponding mRNAs. This is based upon the fact that mRNA hybridized with its complementary DNA will not direct the cell-free synthesis of a complete polypeptide. Full translational activity of the mRNA is recovered upon the heat melting of the hybrid. Utilizing the rabbit beta globin clone PbetaG1, we demonstrate the application of hybrid-arrested translation for the identification of structural gene sequences within recombinant DNA molecules. In addition, the method is used to locate and order precisely several adenovirus 2 polypeptides within the viral genome.

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References

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    1. Biochim Biophys Acta. 1976 Dec 13;454(3):587-91 - PubMed
    1. Virology. 1977 May 15;78(2):522-36 - PubMed
    1. Cell. 1976 Jun;8(2):163-82 - PubMed
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