Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 May 15;26(10):2489-2497.
doi: 10.1016/j.bmcl.2016.03.099. Epub 2016 Mar 30.

Isolation, analytical measurements, and cell line studies of the iron-bryostatin-1 complex

Sydney Plummer  1 , Thomas Manning  2 , Tess Baker  1 , Tysheon McGreggor  1 , Mehulkumar Patel  1 , Greg Wylie  3 , Dennis Phillips  4
Affiliations

Isolation, analytical measurements, and cell line studies of the iron-bryostatin-1 complex

Sydney Plummer et al. Bioorg Med Chem Lett. .

Abstract

Bryostatin-1 is a marine natural product that has demonstrated medicinal activity in pre-clinical and clinical trials for the treatment of cancer, Alzheimer's disease, effects of stroke, and HIV. In this study, iron-bryostatin-1 was obtained using a pharmaceutical aquaculture technique developed by our lab that cultivates marine bacteria for marine natural product extraction. Analytical measurements (1)H and (13)C NMR, mass spectrometry, and flame atomic absorption were utilized to confirm the presence of an iron-bryostatin-1 complex. The iron-bryostatin-1 complex produced was then tested against the National Cancer Institute's 60 cell line panel. Adding iron to bryostatin-1 lowered the anti-cancer efficacy of the compound.

Keywords: Aquaculture; Bryostatin; Bugula neritina; Cancer; Candidatus Endobugula sertula; Cell line; Iron; Synthesis.

PubMed Disclaimer

Publication types

LinkOut - more resources