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Phylogenetic analysis of partial sequences of Zika virus for an imported case of Zika…
Figure
Phylogenetic analysis of partial sequences of Zika virus for an imported case of Zika virus infection in a traveler returning to China from Caracas, Venezuela, February 2016, compared with selected other strains from GenBank. A) Schematic diagram showing the contiguous sequence, obtained from de novo assembly and BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi), targeted at the 3′ terminus of nonstructural protein 2B and the 5′ terminus of nonstructural protein 3 genes (figure not drawn to scale). B) Maximum-likelihood phylogenetic tree inferred based on the Tamura-Nei model (8). The partial sequence of VE_Ganxian (black dot) obtained in this study was analyzed against 11 reference strains of Asian lineage and 5 reference strains of African lineage. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA version 7.0 (http://www.megasoftware.net). GenBank accession numbers are given. Scale bar indicates number of substitutions per site. C, capsid; E, envelope; MTase, methyltransferase; PrM, premembrane; RdRP, RNA-dependent RNA polymerase.
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