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. 1977 Nov;74(11):4969-72.
doi: 10.1073/pnas.74.11.4969.

Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin

M Rubinstein, S Stein, S Udenfriend

Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin

M Rubinstein et al. Proc Natl Acad Sci U S A. 1977 Nov.

Abstract

beta-Endorphin was isolated from 200 rat pituitaries by means of high-performance column chromatography, using sensitive fluorometric methods and a radioreceptor assay for opioid activity. The beta-endorphin was characterized as to molecular weight, amino acid composition, and mapping of tryptic peptides by a new microtechnique. It was found that rat beta-endorphin is identical to camel and sheep beta-endorphin. Furthermore, alpha-endorphin, Met-enkephalin, a nonpeptide morphine-like compound (MLC), and an additional unidentified opioid compound were detected in the extract of rat pituitary.

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References

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    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052-5 - PubMed
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