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. 2015 Jan 15:3:A1-5.
doi: 10.1016/j.bdq.2015.01.001. eCollection 2015 Mar.

qPCR, dPCR, NGS - A journey

Jim F Huggett  1 , Justin O'Grady  2 , Stephen Bustin  3
Affiliations

qPCR, dPCR, NGS - A journey

Jim F Huggett et al. Biomol Detect Quantif. .
No abstract available

Keywords: Digital PCR; MIQE; Next generation sequencing; Reverse transcription; qPCR.

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Figures

Fig. 1
Fig. 1
Publications utilising qPCR published by the time of the first Freising meeting (1996–12/2003). Results from a PubMed search for the terms (1) “real-time PCR” or “realtime PCR” or “real time PCR” or qPCR and (2) “real-time PCR” or “realtime PCR” or “real time PCR” or qPCR and “reverse transcription” were plotted against the year the publication appeared.
Fig. 2
Fig. 2
Publications utilising qPCR published from 2004 to 2014. Results from a PubMed search for the terms (1) “real-time PCR” or “realtime PCR” or “real time PCR” or qPCR and (2) “real-time PCR” or “realtime PCR” or “real time PCR” or qPCR and “reverse transcription” were plotted against the year the publication appeared.
Fig. 3
Fig. 3
Publications utilising dPCR. Results from a PubMed search for the term “digital PCR” were plotted against the year the publication appeared.
Fig. 4
Fig. 4
dPCR analysis of Aspergillus DNA. DNA concentrations of A. fumigatus, A. terreus and A. flavus preparations were measured on a Nanodrop instrument and samples were diluted to 40 fg/μl, and analysed on a Formulatrix Constellation dPCR instrument following 40 cycles of PCR with Agilent Brilliant III mastermix. Hydrolysis probes and primers targeting the 18S rDNA repeat were used, together with a published protocol . (A) A. fumigatus. (B) A. terreus. (C) A. flavus.
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