Phylogeny of Murray Valley encephalitis virus in Australia and Papua New Guinea

Abstract

Objective: To study the genetic diversity of Murray Valley encephalitis virus (MVEV) in Australia and Papua New Guinea.

Methods: MVEV envelope gene sequences were aligned using Clustal X and manual editing was performed with Bioedit. ModelTest v. 3.7 was used to select the simplest evolutionary model that adequately fitted the sequence data. Maximum likelihood analysis was performed using PhyML. The phylogenetic signal of the dataset was investigated by the likelihood mapping analysis. The Bayesian phylogenetic tree was built using BEAST.

Results: The phylogenetic trees showed two main clades. The clade Ⅰ including eight strains isolated from West Australia. The clade Ⅱ was characterized by at least four epidemic entries, three of which localized in Northern West Australia and one in Papua New Guinea. The estimated mean evolutionary rate value of the MVEV envelope gene was 0.407 × 10(-3) substitution/site/year (95% HPD: 0.623 × 10(-4)-0.780 × 10(-3)). Population dynamics defines a relative constant population until the year 2000, when a reduction occurred, probably due to a bottleneck.

Conclusions: This study has been useful in supporting the probable connection between climate changes and viral evolution also by the vector point of view; multidisciplinary monitoring studies are important to prevent new viral epidemics inside and outside new endemic areas.

Keywords: Evolution; Murray valley encephalitis virus; Phylogeny.