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. 2016 Apr;37(4):273-7.
doi: 10.3760/cma.j.issn.0253-2727.2016.04.003.

[Expression of microRNA-191 in T lymphoblastic leukemia/lymphoma and its underlying mechanism]

[Article in Chinese]
Jinghang Zhang  1 , Xaioyu Yang, Min Li, Xin Huang, Cuiling Liu, Zifen Gao
Affiliations

[Expression of microRNA-191 in T lymphoblastic leukemia/lymphoma and its underlying mechanism]

[Article in Chinese]
Jinghang Zhang et al. Zhonghua Xue Ye Xue Za Zhi. 2016 Apr.

Abstract
in English, Chinese

Objective: To evaluate the correlation between MicroRNA-191 (miR-191) and T lymphoblastic leukemia/lymphoma (T-ALL/LBL) to probe its underlying molecular mechanism.

Methods: The expression of miR-191 was examined by real-time PCR (RT-PCR) in 20 T-ALL/LBL tissue samples and 20 lymphoid reactive hyperplasia (LRH) tissue samples. The correlation between miR-191 and the clinicopathological feature of T-ALL/LBL was analyzed. Antisense miR-191 lentiviral vectors was constructed and transfected into T-ALL/LBL Jukat cells. After transfection, the expression of miR-191 was examined by RT-PCR. The cell activity was evaluated by CCK-8 asssy. The cell cycle and apoptosis were determined by flow cytometry.

Results: Compared with LRH samples, the results of RT-PCR showed significant upregulation of miR-191 in 20 T-ALL/LBL tissue samples (1.875±0.079 vs 1.000, P<0.05). The expression level of miR-191 was negatively associated with prognosis. Compared with LV-NC-GFP and control groups, the expression of miR-191 significantly decreased after transfection of antisense miR-191 lentiviral vectors (0.578±0.012 vs 1.011±0.053 and 1.000, P<0.05), the percentages of apoptotic cells and the cell in G0/G1 phase significantly increased (P<0.05).

Conclusions: miR-191 might play a significant role in the development of T-ALL/LBL, implicating a new target for therapy.

目的: 探讨MicroRNA-191（miR-191）与T淋巴母细胞性白血病/淋巴瘤（T-ALL/LBL）的相关性及其作用机制。

方法: 采用荧光实时定量PCR（qRT-PCR）法检测20例T-ALL/LBL患者肿瘤组织和20例淋巴结反应性增生（LRH）患者淋巴结组织中miR-191的表达，并分析其与临床预后的关系。构建反义miR-191慢病毒载体（LV-miR-191-KD）和阴性对照载体（LV-NC-GFP）并转染T-ALL细胞系Jurkat细胞，qRT-PCR法检测miR-191的表达水平。分别用CCK-8法和流式细胞术检测下调miR-191后的细胞活性、细胞周期和凋亡。

结果: T-ALL/LBL患者组miR-191表达水平明显高于LRH患者组（1.875±0.079对1.000，P=0.001），以miR-191表达量的中位数为界，将T-ALL/LBL患者分为高表达组（10例）与低表达组（10例），高表达组患者3年OS率明显低于低表达组（26%对82%，P=0.021）。转染48 h后，LV-miR-191-KD组Jurkat细胞miR-191表达水平（0.578±0.012）较LV-NC-GFP组（1.011±0.053）和未转染对照组（1.000）显著降低（P值分别为0.018和0.021），细胞凋亡比例显著升高（P值均<0.05），细胞周期阻滞于G0/G1期，并抑制从G1期向S期转化。

结论: miR-191在T-ALL/LBL的发生、发展过程中起促进作用，可能作为T-ALL/LBL治疗的潜在靶点。

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Figures

图1
图1. MicroRNA（miR）-191表达对T淋巴母细胞性白血病/淋巴瘤患者总生存的影响
图2
图2. 流式细胞术检测MicroRNA（miR）-191表达对Jurkat细胞凋亡的影响
A：未转染对照组；B：LV-NC-GFP转染组；C：LV-miR-191-KD转染组
图3
图3. CCK-8法检测MicroRNA（miR）-191表达对Jurkat细胞增殖的影响（每组设3个复孔，实验重复3次）
See this image and copyright information in PMC

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