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. 2016 Apr 21:15:26.
doi: 10.1186/s12941-016-0137-1.

Enhancing pathogen identification in patients with meningitis and a negative Gram stain using the BioFire FilmArray(®) Meningitis/Encephalitis panel

Susan H Wootton  1 , Elizabeth Aguilera  1 , Lucrecia Salazar  2 , Andrew C Hemmert  3 , Rodrigo Hasbun  4
Affiliations

Enhancing pathogen identification in patients with meningitis and a negative Gram stain using the BioFire FilmArray(®) Meningitis/Encephalitis panel

Susan H Wootton et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: Meningitis with a negative cerebrospinal (CSF) Gram stain represents a diagnostic and therapeutic challenge. The purpose of our study was to evaluate the performance of the BioFire FilmArray(®) Meningitis/Encephalitis (FA ME) panel in patients presenting with community-acquired meningitis with a negative Gram stain.

Methods: CSF from 48 patients with community-acquired meningitis with a negative Gram stain admitted to four hospitals in Houston, TX underwent additional testing by the FA ME. FA ME results were compared to results obtained as part of routine evaluation.

Results: The panel detected pathogens not previously identified in 11 (22.9 %) of 48, but did not detect pathogens identified by standard technique (West Nile virus, Histoplasma) in 5 (15.2 %) patients.

Conclusions: Rapid testing for the most common pathogens causing meningitis will aid in the diagnosis and treatment of patients with meningitis.

Keywords: Aseptic meningitis; Encephalitis; Molecular diagnostic techniques; Polymerase chain reaction.

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