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. 2016 Apr 22;11(4):e0153752.
doi: 10.1371/journal.pone.0153752. eCollection 2016.

The Clinical Significance of Interleukin-1 Receptor Antagonist +2018 Polymorphism in Rheumatoid Arthritis

Endom Ismail  1 , Omimah Khaled Jaber Nofal  1 , Rajalingham Sakthiswary  2 , Syahrul Sazliyana Shaharir  2 , Radhika Sridharan  3
Affiliations

The Clinical Significance of Interleukin-1 Receptor Antagonist +2018 Polymorphism in Rheumatoid Arthritis

Endom Ismail et al. PLoS One. .

Abstract

Objective: Interleukin-1 receptor antagonist (IL-1Ra) acts as an inhibitor of IL-1; which is one of the culprit cytokines in rheumatoid arthritis (RA). Although +2018 polymorphism of IL-1Ra has been implicated in the pathogenesis of RA, its importance remains poorly understood. Hence, the purpose of this study was to determine the clinical significance of interleukin-1 receptor antagonist (IL-1Ra) +2018 polymorphism in RA.

Methods: Polymerase chain reaction (PCR) and sequencing were used to determine the genotypes of the IL-1Ra +2018 for 77 RA patients and 18 healthy controls. All RA patients were assessed for the disease activity score that includes 28 joints (DAS28) and radiographic disease damage based on Modified Sharp Score (MSS).

Results: The frequency of the T/T and C/T genotypes did not differ significantly (p = 0.893) between the RA patients and the controls. The C/T genotype had significantly higher mean disease activity (DAS 28) and disease damage (MSS) scores with p values of 0.017 and 0.004, respectively. Additionally, the ESR (erythrocyte sedimentation rate), CRP (C-reactive protein), the number of swollen and tender joints were higher for the C/T individuals. On multivariate analysis the CRP, swollen joint count and MSS remained significant with the following p values i.e. 0.045, 0.046 and less than 0.05.

Conclusions: C/T genotype of IL-1Ra +2018 prognosticates more aggressive disease in RA.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

References

    1. Choy EH, Panayi GS. Cytokine pathways and joint inflammation in rheumatoid arthritis. N Engl J Med. 2001. March 22;344(12):907–16. - PubMed
    1. John S, Worthington J. Genetic epidemiology. Approaches to the genetic analysis of rheumatoid arthritis. Arthritis Res. 2001;3(4):216–20. - PMC - PubMed
    1. Cantagrel A, Navaux F, Loubet-Lescoulié P, Nourhashemi F, Enault G, Abbal M, et al. Interleukin-1β, interleukin-1 receptor antagonist, interleukin-4, and interleukin-10 gene polymorphisms: Relationship to occurrence and severity of rheumatoid arthritis. Arthritis & Rheumatism. 1999;42(6):1093–100. - PubMed
    1. Arend WP, Dayer JM. Inhibition of the production and effects of interleukin-1 and tumor necrosis factor alpha in rheumatoid arthritis. Arthritis Rheum. 1995. February;38(2):151–60. - PubMed
    1. Steinkasserer A, Spurr NK, Cox S, Jeggo P, Sim RB. The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14–q21, in the region of the IL-1α and IL-1β loci. Genomics. 1992;13(3):654–7. - PubMed

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