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. 2016:2016:3760191.
doi: 10.1155/2016/3760191. Epub 2016 Mar 24.

A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity

Angeli Kodjo  1 , Christophe Calleja  2 , Michael Loenser  2 , Dan Lin  3 , Joshua Lizer  4
Affiliations

A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity

Angeli Kodjo et al. Biomed Res Int. 2016.

Abstract

A rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a positive cut-off titer of ≥800. Banked serum samples from dogs exhibiting clinical signs and suspected leptospirosis were selected to form three groups based on MAT titer: (1) positive (n = 50); (2) borderline (n = 35); and (3) negative (n = 50). Using an analysis to weight group sizes to reflect French prevalence, the sensitivity and specificity were 98% and 93.5% (88.2% unweighted), respectively. This test rapidly identifies cases of acute canine leptospirosis with high levels of sensitivity and specificity with no interference from previous vaccination.

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References

    1. Levett P. N. Leptospirosis: a forgotten zoonosis? Clinical and Applied Immunology Reviews. 2004;4(6):435–448. doi: 10.1016/j.cair.2004.08.001. - DOI
    1. Blazius R. D., Romão P. R. T., Blazius E. M. C. G., da Silva O. S. Occurrence of Leptospira spp. soropositive stray dogs in Itapema, Santa Catarina, Brazil. Cadernos de Saúde pública. 2005;21(6):1952–1956. doi: 10.1590/s0102-311x2005000600046. - DOI - PubMed
    1. Boutilier P., Carr A., Schulman R. L. Leptospirosis in dogs: a serologic survey and case series 1996 to 2001. Veterinary Therapeutics. 2003;4(2):178–187. - PubMed
    1. Gautam R., Wu C.-C., Guptill L. E., Potter A., Moore G. E. Detection of antibodies against Leptospira serovars via microscopic agglutination tests in dogs in the United States, 2000–2007. Journal of the American Veterinary Medical Association. 2010;237(3):293–298. - PubMed
    1. Geisen V., Stengel C., Brem S., Müller W., Greene C., Hartmann K. Canine leptospirosis infections—clinical signs and outcome with different suspected Leptospira serogroups (42 cases) Journal of Small Animal Practice. 2007;48(6):324–328. doi: 10.1111/j.1748-5827.2007.00324.x. - DOI - PubMed

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