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. 1989 Jan;176(1):157-60.
doi: 10.1016/0003-2697(89)90286-8.

An inexpensive small volume equilibrium dialysis system for protein-ligand binding assays

T Reinard  1 , H J Jacobsen
Affiliations

An inexpensive small volume equilibrium dialysis system for protein-ligand binding assays

T Reinard et al. Anal Biochem. 1989 Jan.

Abstract

A simple and inexpensive system for equilibrium dialysis-based binding assays has been developed employing 1.5-ml microtubes. The main advantages are small volume of test buffers, no time-consuming assembly of the test vials, and inexpensive test cells. A comparison of the results derived from this equilibrium dialysis system with those of polyethylenimine-filtration assay and those of a commercially available equilibrium dialysis test system (Diachema from Dianorm) shows good agreement.

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