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. 2016 Apr 28;16(1):41.
doi: 10.1093/jisesa/iew025. Print 2016.

Lyophilized artificial diet for rearing the Neotropical Euschistus heros (Hemiptera: Pentatomidae)

Agustín C Mendoza  1 , Aline C P da Rocha  2 , José R P Parra  2
Affiliations

Lyophilized artificial diet for rearing the Neotropical Euschistus heros (Hemiptera: Pentatomidae)

Agustín C Mendoza et al. J Insect Sci. .

Abstract

An artificial diet to mass-rear Euschistus heros (F. 1798) (Hemiptera: Pentatomidae) was developed in the laboratory. Biological studies were conducted under controlled conditions of temperature (25 ± 2 °C), RH: 60 ± 10%, and photoperiod of 14:10 (L:D) h. Out of 13 diets tested, 2 diets (D9 and D11) were the most suitable. The artificial diets selected had the same composition (green beans, peanuts, sucrose, water, Nipagin, and sorbic acid) except for different antimicrobial agents (D11 has tetracycline, and D9 doesn't). The 68% viability for the egg-adult period of insects reared on these lyophilized artificial diets (LAD) was almost twice as high as the 38% viability obtained with the natural diet. Although adults reared on LAD weighed 17% less than those reared on the natural diet, mean fecundity was higher than on the natural diet (282 eggs/female), reaching 430 eggs/female. The net reproductive rate (Ro) increased over the generations for the diets with lyophilized material and antimicrobial agents. The opposite occurred with the diet of lyophilized material without antimicrobial agents, showing that the insects either adapted or degenerated through generations. Lyophilized diets supported the production of E. heros through at least 10 generations, with no degeneration.

Keywords: Soybean brown stink bug; anticontaminant; lyophilized diet.

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Figures

Fig. 1.
Fig. 1.
Flowchart of steps designed to select lyophilized artificial diet for E. heros rearing.
Fig. 2.
Fig. 2.
UPGMA Phenogram for mean Euclidean distance of four steps in the improvement of the artificial diet containing lyophilized green beans (LAD) and antimicrobial agents for E. heros. Temp.: 25 ± 2°C; RH: 60 ± 10%; photoperiod of 14:10 (L:D) h. D1* in steps 3 and 4, the mean values determined in experiment 1 and steps 1 and 2 were used.
See this image and copyright information in PMC

References

    1. Alverson J., Cohen A. C. 2002. Effect of antifungal agents on biological fitness of Lygus hesperus (Heteroptera: Miridae). J. Econ. Entomol. 95: 256–260. - PubMed
    1. Corrêa-Ferreira B. S. 1993. Utilização do parasitoide de ovos Trissolcus basalis (Wollaston) no controle de percevejo da soja, p. 40. In Circular Técnica, 11, Embrapa-CNPSo, Londrina, PR.
    1. Douglas G. I., Sikorowski P. P. 2009a. Microbial contamination and insect rearing, pp. 150–217. In Schneider J. C. (ed.), Principles and Procedures for Rearing High Quality Insects. Mississippi State University, Starkville, MS, USA.
    1. Douglas G. I., Sikorowski P. P. 2009b. Entomopathogens and insect rearing, pp. 224–288. In Schneider J. C. (ed.), Principles and Procedures for Rearing High Quality Insects. Mississippi State University, Starkville, MS, USA.
    1. Fortes P., Magro S. R., Panizzi A. R., Parra J. R. P. 2006. Development of a dry artificial diet for Nezara viridula (L.) and Euschistus heros (Fabricius) (Heteroptera: Pentatomidae). Neotrop. Entomol. 35: 567–572. - PubMed