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. 1989 Feb;55(2):355-8.
doi: 10.1016/S0006-3495(89)82812-7.

Two-dimensional 1H/13C heteronuclear chemical shift correlation spectroscopy of lipid bilayers

C W Lee  1 , R G Griffin
Affiliations

Two-dimensional 1H/13C heteronuclear chemical shift correlation spectroscopy of lipid bilayers

C W Lee et al. Biophys J. 1989 Feb.

Abstract

Using solid-state magic angle spinning nuclear magnetic resonance (NMR) techniques, we have obtained two-dimensional (2D), 1H/13C chemical shift-correlated spectra of liquid crystalline 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) bilayers in 30 wt% PO4/D2O buffer. Linewidths in both the 13C and the 1H dimensions were less than 0.3 ppm wide. The 2D spectrum consists of chemical shift correlations between all resolvable, directly bonded 13C-1H pairs and exhibits considerably greater spectral dispersion than either ID 1H or 13C MAS spectra. This approach promises to be an important tool in structural studies of biological membranes.

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