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. 2015 Sep;23(4):421-8.
doi: 10.1016/j.jsps.2014.11.007. Epub 2014 Nov 26.

Synthesis and characterization of biocompatibility of tenorite nanoparticles and potential property against biofilm formation

Davoodbasha MubarakAli  1 , Jegatheesan Arunkumar  2 , Pratheesh Pooja  3 , Gopalakrishnan Subramanian  3 , Nooruddin Thajuddin  4 , Naiyf S Alharbi  5
Affiliations

Synthesis and characterization of biocompatibility of tenorite nanoparticles and potential property against biofilm formation

Davoodbasha MubarakAli et al. Saudi Pharm J. 2015 Sep.

Abstract

Aim is to assess the anti-biofilm property of tenorite nanoparticles and to study their suitability as a possible coating material for medical implants. Tenorite (CuO) nanoparticles were synthesized by the optimized thermal decomposition method and characterized using TEM, XRD, FTIR and UV-Vis analysis. Their influence on biofilm formation of microbes was studied by growing multi drug resistant bacterial strains in the presence or absence of these nanoparticles at various concentrations. The cytotoxicity of nanoparticles on mammalian cells was studied at the corresponding concentrations. The nanoparticles were found to be uniformly dispersed, spherical shaped and <50 nm in size. They showed various degrees of anti-biofilm property against clinically isolated, biofilm forming multi drug resistant microorganisms such as Staphylococcus aureus, Pseudomonas fluorescens, Burkholderia mallei, Klebsiella pneumoniae, and Escherichia coli. Furthermore, Hep-2 cells showed excellent viability at tenorite nanoparticles concentration toxic to microbial growth. These results indicate that tenorite nanoparticles may be ideal candidates for being utilized as coating on medical implants in general and dental implants in particular.

Keywords: Antibiofilm; Cell viability; Implants; Multi drug resistant; TEM; Tenorite nanoparticle.

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Figures

Figure 1
Figure 1
UV–Vis spectrum of tenorite nanoparticles showed Plasmon resonance at 550 nm.
Figure 2
Figure 2
FTIR spectrum recorded for tenorite nanoparticle, the bands seen at 1115.4 and 668.77 cm−1 were assigned to the metallic and O stretching vibrations of the metallic oxides respectively.
Figure 3
Figure 3
XRD analysis of synthesized tenorite nanoparticles showed (1 1 1), (0 0 2), (1 1 0) planes corresponding to the crystalline nanoparticles of about 17 nm.
Figure 4
Figure 4
TEM micrograph of tenorite nanoparticles showed the average size of <50 nm was spherical in shape.
Figure 5
Figure 5
Light microscopic images of biofilm formed by Klebsiella pneumoniae (A), Escherichia coli (B) and Staphylococcus aureus MTCC96 (C) and (A1; B1;C1) treated with tenorite nanoparticles (32 μM).
Figure 6
Figure 6
Cytotoxicity of tenorite nanoparticles with Hep-2 cell lines (A–D); cytologically tenorite nanoparticles showed toxicity up to the concentration of (650 μM–162 μM) and viability from 81 μM to 5 μM (E–H).
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