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Case Reports
. 2016 Jan-Feb;6(1):56-8.
doi: 10.4103/2141-9248.180275.

Use of an Innovative Simple Method for Anaerobiosis in the Diagnosis and Management of Infections in Two Unusual Cases

J Haldar  1 , N Pal  1 , R Ray  1 , P K Maiti  1
Affiliations
Case Reports

Use of an Innovative Simple Method for Anaerobiosis in the Diagnosis and Management of Infections in Two Unusual Cases

J Haldar et al. Ann Med Health Sci Res. 2016 Jan-Feb.

Abstract

Technical limitations restrict routine anaerobe isolation from clinical materials in resource-limited laboratories. An innovative two steps combustion candle jar technique may be suitable for such setup. This system was tried with one case of chronic osteomyelitis developed on supracondyler compound fracture. Porphyromonas spp. was isolated and identified. Vancomycin was recommended based on in vitro sensitivity test, but the leg was amputed after receiving a resistant drug gentamycin. While in another child with hydrocephalous, V-P shunt associated infection by Peptostreptococcus anaerobius was successfully controlled by sensitive drug vancomycin. These two eye-opener cases insisted us for large scale application of the technique.

Keywords: Anaerobiosis; Candle-jar technique; Osteomyelitis; Porphyromonas spp; V-P shunt infection.

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Figures

Figure 1
Figure 1
Brown pigmented colonies of Porphyromona spp. On enriched Brucella blood agar medium
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