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. 2016 Apr 11;4(4):apps.1500095.
doi: 10.3732/apps.1500095. eCollection 2016 Apr.

Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)

Joanna Weremijewicz  1 , Jasmin I Almonte  2 , Vanessa S Hilaire  2 , Frank D Lopez  2 , Stephen H Lu  2 , Sarah M Marrero  2 , Catherine M Martinez  2 , Edson A Zarate  2 , Ana K Lam  2 , Samantha A N Ferguson  2 , Nicolas Z Petrakis  2 , Kelsey A Peeples  2 , Ebony D Taylor  2 , Natalie M Leon  2 , Carolina Valdes  2 , Michael Hass  2 , Andrew B Reeve  2 , Danielle T Palow  2 , Jason L Downing  3
Affiliations

Microsatellite primers for two threatened orchids in Florida: Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae)

Joanna Weremijewicz et al. Appl Plant Sci. .

Abstract

Premise of the study: The Million Orchid Project at Fairchild Tropical Botanic Garden is an initiative to propagate native orchids for reintroduction into Miami's urban landscapes. The aim of this study was to develop microsatellites for Encyclia tampensis and Cyrtopodium punctatum (Orchidaceae).

Methods and results: Ten microsatellites were developed for each species. For E. tampensis sampled from the natural population, allele numbers ranged from one to four, with an average observed heterozygosity (H o) of 0.314 and average expected heterozygosity (H e) of 0.281. For the individuals from cultivation, allele numbers ranged from one to six, with an average H o of 0.35 and an average H e of 0.224. For C. punctatum, allele numbers ranged from one to three, with an average H o of 0.257 and an average H e of 0.272.

Conclusions: These microsatellites will be used to assess the genetic diversity of natural and cultivated populations with the intention of guiding genetic breeding under the Million Orchid Project.

Keywords: Cyrtopodium punctatum; Encyclia tampensis; Orchidaceae; commercially exploited plants; microsatellites; native Florida orchids.

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References

    1. Castoe T. A., Poole A. W., de Koning A. P. J., Jones K. L., Tomback D. F., Oyler-McCance S. J., Fike J. A., et al. 2012. Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and one snake. PLoS ONE 7: e30953. - PMC - PubMed
    1. Chafin L. G. 2000. Field guide to the rare plants of Florida. Florida Natural Areas Inventory, Tallahassee, Florida. Website http://www.fnai.org/FieldGuide/pdf/Cyrtopodium_punctatum.pdf [accessed 24 June 2015].
    1. Dutra D., Kane M. E., Reinhardt Adams C., Richardson L. 2009. Reproductive biology of Cyrtopodium punctatum in situ: Implications for conservation of an endangered Florida orchid. Plant Species Biology 24: 92–103.
    1. Emerson E. 2010. Hardy-Weinberg equilibrium calculator for 3 alleles. Website http://www.had2know.com/academics/hardy-weinberg-equilibrium-calculator-... [accessed 1 July 2015].
    1. Koressaar T., Remm M. 2007. Enhancements and modifications of primer design program Primer3. Bioinformatics (Oxford, England) 23: 1289–1291. - PubMed

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