Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 May 4:68:2.24.1-2.24.15.
doi: 10.1002/cptx.1.

Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay

Filomena S G Silva  1   2 , Irina G Starostina  3   2 , Vilena V Ivanova  3 , Albert A Rizvanov  3 , Paulo J Oliveira  1 , Susana P Pereira  4
Affiliations

Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay

Filomena S G Silva et al. Curr Protoc Toxicol. .

Abstract

The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in the same cell sample using a dual resazurin/sulforhodamine B assay, eliminating the variation associated with cell seeding and excessive manipulations in assays that test different cell samples across plates. The procedure also reduces the amount of cells, test compound, and reagents required, as well as the time expended in conventional tests, thus resulting in a more confident prediction of toxic thresholds for the tested compounds. © 2016 by John Wiley & Sons, Inc.

Keywords: high throughput; human skin fibroblasts; preclinical tests; toxicity evaluation.

PubMed Disclaimer

References

Literature Cited

    1. Abdoli, N. , Azarmi, Y. , and Eghbal, M.A. 2015. Mitigation of statins-induced cytotoxicity and mitochondrial dysfunction by L-carnitine in freshly-isolated rat hepatocytes. Res. Pharm. Sci. 10:143-151.
    1. Adams, C.P. and Brantner, V.V. 2006. Estimating the cost of new drug development: Is it really 802 million dollars? Health Aff. 25:420-428. doi: 10.1377/hlthaff.25.2.420.
    1. Ahmed, S.A. , Gogal, R.M., Jr. , and Walsh, J.E. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to [3H]thymidine incorporation assay. J. Immunol. Methods 170:211-224. doi: 10.1016/0022-1759(94)90396-4.
    1. Allen, D.D. , Caviedes, R. , Cardenas, A.M. , Shimahara, T. , Segura-Aguilar, J. , and Caviedes, P.A. 2005. Cell lines as in vitro models for drug screening and toxicity studies. Drug Dev. Ind. Pharm. 31:757-768. doi: 10.1080/03639040500216246.
    1. Ambrosi, G. , Ghezzi, C. , Sepe, S. , Milanese, C. , Payan-Gomez, C. , Bombardieri, C.R. , Armentero, M.T. , Zangaglia, R. , Pacchetti, C. , Mastroberardino, P.G. , and Blandini, F. 2014. Bioenergetic and proteolytic defects in fibroblasts from patients with sporadic Parkinson's disease. Biochim. Biophys. Acta 1842:1385-1394. doi: 10.1016/j.bbadis.2014.05.008.

Publication types

LinkOut - more resources