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. 2016 Feb 20;16(2):e32427.
doi: 10.5812/hepatmon.32427. eCollection 2016 Feb.

Association Between IL-10 Gene Promoter Polymorphisms (-592 A/C, -819 T/C, -1082 A/G) and Susceptibility to HBV Infection in an Iranian Population

Bita Moudi  1 , Zahra Heidari  1 , Hamidreza Mahmoudzadeh-Sagheb  1 , Mohammad Hashemi  2 , Malihe Metanat  3 , Soheila Khosravi  4 , Parisa Farrokh  5
Affiliations

Association Between IL-10 Gene Promoter Polymorphisms (-592 A/C, -819 T/C, -1082 A/G) and Susceptibility to HBV Infection in an Iranian Population

Bita Moudi et al. Hepat Mon. .

Abstract

Background: IL-10 can play a vital role in immune response against HBV. Three biallelic SNPs from the transcription start site control the transcription of the IL-10 gene. An association between susceptibility to HBV and IL-10 polymorphisms has been suggested in patients with HBV infection.

Objectives: The present study was designed to study the association between polymorphisms in interleukin-10 (-1082 A/G, -819 T/C and -592 A/C) promoter gene and chronic hepatitis B virus (HBV) infection.

Patients and methods: 221 chronically infected patients and 200 healthy control subjects were enrolled in the study. Three biallelic (-1082 A/G, -819 T/C and -592 A/C) polymorphisms in the IL-10 promoter gene were determined by PCR-RFLP method.

Results: Persistent HBV infection was associated with IL-10-1082 AG (P = 0.001) and GG (P = 0.004) genotypes and G (P = 0.000) allele. IL-10-819 T/C and -592 A/C genotype and allele frequencies did not show any correlation with the risk of chronic hepatitis B infection.

Conclusions: These results suggest that polymorphisms in interleukin-10 gene promoter influence clinical outcome of HBV infection and susceptibility to HBV infection.

Keywords: Chronic Hepatitis B; Interleukin-10; Single Nucleotide Polymorphism.

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Figures

Figure 1.
Figure 1.. Agarose Gel Electrophoresis Illustrating PCR Products for the IL-10 Promoter Polymorphism (IL10-592 A/C SNP)
Lane 1, marker; lanes 2 and 5, homozygous CC subjects (C allele does not digest with RSaI); lanes 3 and 6, heterozygous AC subjects; lanes 4 and 7, homozygous AA subjects (A allele digests with RSaI to produce 236 bp and 176 bp fragments).
Figure 2.
Figure 2.. Agarose Gel Electrophoresis Illustrating PCR Products for the IL-10 Promoter Polymorphism (IL10-819 T/C SNP)
Lane 1, marker; lanes 2 and 5, homozygous CC subjects (C allele digests with RseI to produce 443 bp and 116 bp fragments); lanes 3 and 6, heterozygous TC subjects; lanes 4 and 7, homozygous TT subjects (T allele does not digest with RseI).
Figure 3.
Figure 3.. Agarose Gel Electrophoresis Illustrating PCR Products for the IL-10 Promoter Polymorphism (IL10-1082 A/G SNP)
Lane 1, marker; lanes 2 and 5, homozygous AA subjects (A allele digests with MnlI to produce 134 bp and 65 bp fragments); lanes 3 and 6, heterozygous TC subjects; lanes 4 and 7, homozygous GG subjects (G allele digests with MnlI to produce 112 bp and 65 bp and 22 bp fragments).
See this image and copyright information in PMC

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