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. 2016 May 4:11:20.
doi: 10.1186/s13027-016-0067-8. eCollection 2016.

Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in São Paulo, Brazil

Julio C Betiol #  1 , Laura Sichero #  1   2 , Henrique O de Olival Costa  3 , Leandro L de Matos  4 , Maria A Andreoli  5 , Silvaneide Ferreira  1 , Sheila F Faraj  6 , Evandro S de Mello  6 , João S Sobrinho  1 , Lenine G Brandão  4 , Claudio R Cernea  4 , Marco A Kulcsar  4 , Fabio R Pinto  4 , Antonio J Gonçalves  7 , Marcelo B Menezes  7 , Leonardo Silva  3 , Lia M Rossi  3 , Rafaella A Lima Nunes  5 , Lara Termini  5 , Luisa L Villa  1   5   8
Affiliations

Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in São Paulo, Brazil

Julio C Betiol et al. Infect Agent Cancer. .

Abstract

Background: Human papillomavirus (HPV) prevalence in head and neck squamous cell carcinomas (HNSCC) diverges geographically. The reliability of using p16(INK4a) expression as a marker of viral infection is controversial in HNSCC. We evaluated HPV types and HPV-16 variants prevalence, and p16(INK4a) expression in HNSCC specimens provided by two different Institutions in São Paulo.

Methods: HPV DNA from formalin-fixed specimens was accessed by Inno-LiPA, HPV-16 variants by PCR-sequencing, and p16(INK4a) protein levels by immunohistochemistry.

Results: Overall, HPV DNA was detected among 19.4 % of the specimens (36/186). Viral prevalence was higher in the oral cavity (25.0 %, 23/92) then in other anatomical sites (oropharynx 14,3 %, larynx 13.7 %) when samples from both Institutions were analyzed together. HPV prevalence was also higher in the oral cavity when samples from both Institutions were analyzed separately. HPV-16 was the most prevalent type identified in 69.5 % of the HPV positive smaples and specimens were assigned into Asian-American (57.2 %) or European (42.8 %) phylogenetic branches. High expression of p16(INK4a) was more common among HPV positive tumors.

Conclusion: Our results support a role for HPV-16 in a subset of HNSCC.

Keywords: Head and neck cancer; Human papillomavirus; Molecular variants; Prevalence; p16INK4a.

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Figures

Fig. 1
Fig. 1
Overall HPV DNA prevalence by anatomical subsite and sample origin. Prevalence (%) and 95 % CI are indicated. FCMSCSP-Santa Casa de Sao Paulo, School of Medicine; ICESP-Cancer Institute of São Paulo
See this image and copyright information in PMC

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