Soil metaproteomics reveals an inter-kingdom stress response to the presence of black truffles

Abstract

For some truffle species of the Tuber genus, the symbiotic phase is often associated with the presence of an area of scant vegetation, commonly known as the brûlé, around the host tree. Previous metagenomics studies have identified the microorganisms present inside and outside the brûlé of a Tuber melanosporum truffle-ground, but the molecular mechanisms that operate in this ecological niche remain to be clarified. To elucidate the metabolic pathways present in the brûlé, we conducted a metaproteomics analysis on the soil of a characterized truffle-ground and cross-referenced the resulting proteins with a database we constructed, incorporating the metagenomics data for the organisms previously identified in this soil. The soil inside the brûlé contained a larger number of proteins and, surprisingly, more proteins from plants, compared with the soil outside the brûlé. In addition, Fisher's Exact Tests detected more biological processes inside the brûlé; these processes were related to responses to multiple types of stress. Thus, although the brûlé has a reduced diversity of plant and microbial species, the organisms in the brûlé show strong metabolic activity. Also, the combination of metagenomics and metaproteomics provides a powerful tool to reveal soil functioning.

Figure 1. Picture of a brûlé in the Cahors truffle-ground.

Figure 2. Schematic representation of the protein extraction methods.

Figure 3. Categorization by Gene Ontology analysis into biological processes (a) and in molecular functions (b) having a minimum of 100 proteins identified.

Figure 4. Categorization by Gene Ontology analysis in biological processes level 3 (a) and in molecular functions level 3 (b) of the T. melanosporum proteins.

Figure 5. Schematic representation of the proteins and the processes detected inside and outside the T. melanosporum brûlé.