doi: 10.1038/339237a0.
Avoiding false positives with PCR
Affiliations
- PMID: 2716852
- DOI: 10.1038/339237a0
Item in Clipboard
Avoiding false positives with PCR
Nature.
.
Erratum in
- Nature 1989 Jun 8;339(6224):490
Abstract
The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.
Similar articles
-
Setting up a polymerase chain reaction laboratory.Methods Mol Biol. 2006;336:11-8. doi: 10.1385/1-59745-074-X:11. Methods Mol Biol. 2006. PMID: 16916249 Review.
-
Contaminating insert degradation by preincubation colony PCR: a method for avoiding false positives in transformant screening.Anal Biochem. 2008 Apr 1;375(1):159-61. doi: 10.1016/j.ab.2007.11.029. Epub 2007 Nov 28. Anal Biochem. 2008. PMID: 18164254
-
PCR diagnosis of tuberculosis--experience in India.Indian J Pediatr. 2002 Nov;69 Suppl 1:S20-4. Indian J Pediatr. 2002. PMID: 12501921
-
Cost effectiveness of testing strategies for chronic hepatitis C.Am J Gastroenterol. 2005 Mar;100(3):607-15. doi: 10.1111/j.1572-0241.2005.40531.x. Am J Gastroenterol. 2005. PMID: 15743359
-
[Molecular methods in the diagnosis of infectious diseases].Przegl Epidemiol. 2003;57(2):381-9. Przegl Epidemiol. 2003. PMID: 12910610 Review. Polish.
Cited by
-
Metabolic/hypoxial axis predicts tamoxifen resistance in breast cancer.Sci Rep. 2022 Sep 27;12(1):16118. doi: 10.1038/s41598-022-19977-w. Sci Rep. 2022. PMID: 36167713 Free PMC article.
-
Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.J Clin Microbiol. 1992 Apr;30(4):845-53. doi: 10.1128/jcm.30.4.845-853.1992. J Clin Microbiol. 1992. PMID: 1572969 Free PMC article.
-
Repeated polymerase chain reaction complementary to other conventional methods for early detection of HIV infection in infants born to HIV-infected mothers.Eur J Clin Microbiol Infect Dis. 1991 Mar;10(3):146-56. doi: 10.1007/BF01964447. Eur J Clin Microbiol Infect Dis. 1991. PMID: 2060515
-
Detection of replicative intermediates of hepatitis C viral RNA in liver and serum of patients with chronic hepatitis C.J Clin Invest. 1991 Sep;88(3):1058-60. doi: 10.1172/JCI115368. J Clin Invest. 1991. PMID: 1653272 Free PMC article.
-
Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients.J Clin Microbiol. 1992 Feb;30(2):527-30. doi: 10.1128/jcm.30.2.527-530.1992. J Clin Microbiol. 1992. PMID: 1311338 Free PMC article.
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
