Detection of Torque teno midi virus/Small anellovirus (TTMDV/SAV) in the sera of domestic village chickens and its vertical transmission from hen to eggs

Abstract

Although the infection of different animals and non-human primates with other members of Anelloviridae have already been reported there is no report about infection of animals with Torque teno midi virus/Small anellovirs (TTMDV/SAV). The aim of this study was to detect the virus in domestic village chickens. Blood samples were collected from 79 domestic village chickens in Isfahan. Blood samples of five adult laying hens and one cockerel were collected in three consecutive weeks (days 1, 8 and 14) as experimental chickens. Ten eggs were randomly collected from the eggs laid during days 12 to 17 and thin and thick egg whites and yolk samples were collected aseptically. After DNA extraction Nested-PCR was performed using SMAs/SMAr primers. In PCR, 431 bp and 441 bp products were detected. The detected bands were extracted and sequenced. Totally 26 out of 79 (32.9%) of the blood samples were positive for the virus. The frequency of the infection of the different parts of the eggs tested was 76%. For the first time TTMDV/SAV was detected in domestic village chickens which also vertically transmitted to eggs.

Keywords: Domestic village chickens; Torque teno midi virus/Small anellovirus (TTMDV/SAV); Vertical transmission.

Fig. 1

Agarose gel electrophoresis showing PCR products of tested samples (1: Thin white, 2: Thick white, 3: Yolk, S1-S5: Serum samples, M: Marker; C⁺, Positive control, and C^-: Negative control

Fig. 2

Phylogenetic tree constructed against Torque teno midi virus species 1, 2, and 3 sequences of the genus Gamma torquevirus of Anelloviridae. The sequences obtained in this study are shown in bold text

Fig. 3

Phylogenetic tree constructed against the sequences already obtained from human samples in our laboratory. The sequences obtained in this study are shown in bold text