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. 2015 Spring;16(2):218-22.

Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis

A Awad  1 , S R Khalil  2 , Y M Abd-Elhakim  2
Affiliations

Molecular phylogeny of some avian species using Cytochrome b gene sequence analysis

A Awad et al. Iran J Vet Res. 2015 Spring.

Abstract

Veritable identification and differentiation of avian species is a vital step in conservative, taxonomic, forensic, legal and other ornithological interventions. Therefore, this study involved the application of molecular approach to identify some avian species i.e. Chicken (Gallus gallus), Muskovy duck (Cairina moschata), Japanese quail (Coturnix japonica), Laughing dove (Streptopelia senegalensis), and Rock pigeon (Columba livia). Genomic DNA was extracted from blood samples and partial sequence of the mitochondrial cytochrome b gene (358 bp) was amplified and sequenced using universal primers. Sequences alignment and phylogenetic analyses were performed by CLC main workbench program. The obtained five sequences were deposited in GenBank and compared with those previously registered in GenBank. The similarity percentage was 88.60% between Gallus gallus and Coturnix japonica and 80.46% between Gallus gallus and Columba livia. The percentage of identity between the studied species and GenBank species ranged from 77.20% (Columba oenas and Anas platyrhynchos) to 100% (Gallus gallus and Gallus sonneratii, Coturnix coturnix and Coturnix japonica, Meleagris gallopavo and Columba livia). Amplification of the partial sequence of mitochondrial cytochrome b gene proved to be practical for identification of an avian species unambiguously.

Keywords: Avian species; Cytochrome b gene; Phylogenetic analysis.

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Figures

Fig. 1
Fig. 1
Electrophoretic analysis of PCR product amplified with mt Cytb universal primer. Lane M: 100 bp plus DNA marker, Lane 1: Chicken (Gallus gallus), Lane 2: Japanese quail (Coturnix japonica), Lane 3: Muskovy duck (Cairina moschata), Lane 4: Laughing dove (Streptopelia senegalensis), and Lane 5: Rock pigeon (Columba livia)
Fig. 2
Fig. 2
Alignment of the partial sequence of mitochondrial cytochrome b (mt Cytb) gene from chicken (Gallus gallus), Japanese quail (Coturnix japonica), muskovy duck (Cairina moschata), laughing dove (Streptopelia senegalensis) and rock pigeon (Columba livia). Sequences were aligned using CLC Main Workbench program. Dots indicate sequence identity
Fig. 3
Fig. 3
Phylogenetic analysis based on the cytochrome b gene sequences of five avian species and other related species. The tree was analyzed by neighbor-joining (N-J) analysis
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