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Comment
. 2016 May;14(2):206-11.
doi: 10.2450/2016.0209-15. Epub 2016 Mar 3.

Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured on a scaffold made of silk fibroin and cord blood platelet gel

Anna L M Ferri  1 , Valentina Ceserani  1 , Noemi Greppi  2 , Valentina Tosetti  3 , Marco Schiariti  4 , Giulio Alessandri  3 , Paolo Rebulla  2 , Eugenio Parati  3
Affiliations
Comment

Osteogenic differentiation of adipose tissue-derived mesenchymal stem cells cultured on a scaffold made of silk fibroin and cord blood platelet gel

Anna L M Ferri et al. Blood Transfus. 2016 May.
No abstract available

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Figures

Figure 1
Figure 1
Schematic representation of the ADMSC co-culture system in the presence of a SF/CBPG scaffold. A 12-well co-culture system (Nunc) was used. ADMSC were placed on the bottom layer and cultured in DMEM plus 0.2% BSA. In the upper layer we placed the SF/CBPG scaffold (column A), CBPG (column B) and SF (column C). A control in which ADMSC were cultured in standard conditions (DMEM +10% FBS) was also prepared (column D). After the first 4 days we observed a reduction of cell growth and the formation of 3D multicellular spheroids both in cells cultured with the SF/CBPG scaffold and CBPG. The analysis was repeated three times on six different ADMSC lines. ADMSC: adipose mesenchymal stem cells; SF/CBPG: silk fibroin/cord blood platelet gel; DMEM: standard culture medium.
Figure 2
Figure 2
Analysis of alizarin red staining. Top left: alizarin red staining was performed to confirm the presence of mineral matrix deposition in ADMSC cultured in the presence of SF/CBPG or CBPG. Top right: equal amounts of cDNA isolated from ADMSC co-cultured for 6 days with SF/CBPG or CBPG were amplified for RUNX2, COL1, Osteopontin and Osteocalcin and compared to that obtained from ADMSC cultured in standard conditions. Interestingly, gene expression was significantly higher in SF/CBPG-cultured cells. Bottom: a real-time PCR assay showed the Notch cascade upregulation involved in the osteogenic differentiation of ADMSC cultured in the presence of SF/CBPG or CBPG. ADMSC: adipose mesenchymal stem cells; SF/CBPG: silk fibroin/cord blood platelet gel; DMEM: standard culture medium.
Figure 3
Figure 3
Evaluation of the osteogenic potential of the PL. Comparison of ADMSC cultured in standard conditions (left) and in the presence of PL (right). ADMSC had a comparable growth rate in the two media up to 12 passages. Different concentrations of FBS and PL were tested. Interestingly, there was no formation of any 3D multicellular spheroids as previously observed in SF/CBPG conditions. ADMSC: adipose mesenchymal stem cells; FBS: foetal bovine serum; PL: platelet lysate.
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References

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