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. 2016 Jan;22(1):143-51.
doi: 10.1007/s12298-016-0337-3. Epub 2016 Jan 16.

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

R Thaniarasu  1 , T Senthil Kumar  2 , M V Rao  1
Affiliations

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

R Thaniarasu et al. Physiol Mol Biol Plants. 2016 Jan.

Abstract

The present study describes the plant propagation via indirect organogenesis from in vitro derived leaf and internode explants of Plectranthus bourneae, an endemic plant to south India. Leaf and internodal explants successfully callused on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins [2,4-D (2,4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid) and PIC (Picloram); 0.1-2.0 mg/l] in combination with BA (6-benzyladenine) (0.5 mg/l). Maximum callus induction (98 %) was achieved from leaf explant followed by internodal explant (89 %) at 1.0 mg/l NAA, 0.5 mg/l BA. Leaf derived callus showed better shoot regeneration (29.71 shoots) on MS medium containing 1.0 mg/l KN (kinetin), 0.7 mg/l NAA, and 50 mg/l CH (casein hydrolysate) followed by internodal callus (19.71). A maximum of 19.14 roots/shoot was observed at 1.0 mg/l IBA. The rooted plantlets were successfully hardened and transferred to greenhouse condition with 80 % survival. This system could be utilized for large-scale multiplication of P. bourneae by tissue culture.

Keywords: Callus induction; Endemic plant; Growth regulators; Organogenesis; Plectranthus bourneae.

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Figures

Fig. 1
Fig. 1
Callus induction and plant regeneration from leaf and internode explants of P. bourneae. a leaf explant showing callus initiation, after 2 weeks of inoculation, b Callus proliferation on MS + NAA (1.0 mg/l) + BA (0.5 mg/l), after 4 weeks, c Shoot proliferation on MS + KN (1.0 mg/l), after 4 weeks of culture, d Internode explant showing callus initiation, after 2 weeks of inoculation, e Callus proliferation on MS + NAA (1.0 mg/l) + BA (0.5 mg/l), after 4 weeks, f Shoot proliferation on MS + KN (1.0 mg/l), after 4 weeks of culture, g & h Shoot multiplication and elongation on MS + KN (1.0 mg/l) + NAA (0.7 mg/l) + CH (50 mg/l) from internode and leaf derived callus, after 6 weeks of culture, i Rooting on MS + IBA (1.0 mg/l), after 3 weeks, j Hardening of in vitro raised plantlets, after 1 month. The bar represents 1.0 cm in a-f, 1.0 cm in g, 1.5 cm in h, 0.4 cm in i and 1.0 cm in j
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