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. 2016 Apr-Jun;6(2):112-6.

Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells

Zahra Arab-Bafrani  1 , Daryoush Shahbazi-Gahrouei  2 , Mahdi Abbasian  3 , Mehrafarin Fesharaki  4
Affiliations

Multiple MTS Assay as the Alternative Method to Determine Survival Fraction of the Irradiated HT-29 Colon Cancer Cells

Zahra Arab-Bafrani et al. J Med Signals Sens. 2016 Apr-Jun.

Abstract

A multiple colorimetric assay has been introduced to evaluate the proliferation and determination of survival fraction (SF) of irradiated cells. The estimation of SF based on the cell-growth curve information is the major advantage of this assay. In this study, the utility of multiple-MTS assay for the SF estimation of irradiated HT-29 colon cancer cells, which were plated before irradiation, was evaluated. The SF of HT-29 colon cancer cells under irradiation with 9 MV photon was estimated using multiple-MTS assay and colony assay. Finally, the correlation between two assays was evaluated. Results showed that there are no significant differences between the SF obtained by two assays at different radiation doses (P > 0.05), and the survival curves have quite similar trends. In conclusion, multiple MTS-assay can be a reliable method to determine the SF of irradiated colon cancer cells that plated before irradiation.

Keywords: Colon cancer cells; colony assay; multiple-MTS assay; plating before irradiation.

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Figures

Figure 1
Figure 1
Schematic cross-section of the cell irradiation phantom
Figure 2
Figure 2
The growth curve of cells based on multiple-MTS assay and delay in cell growth of irradiated cells (dash line: a [2 Gy], b [4 Gy], c [6 Gy], d [8 Gy]) versus control cells (solid line: 0 Gy)
Figure 3
Figure 3
(a) Survival curve obtained from the implementation of colonogic and multiple MTS-assay. (b) Correlation of both mentioned methods
See this image and copyright information in PMC

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