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. 2016 Apr;10(4):LC05-9.
doi: 10.7860/JCDR/2016/18877.7627. Epub 2016 Apr 1.

Effect of Probiotic Dietary Intervention on Calcium and Haematological Parameters in Geriatrics

Manisha Kalpesh Gohel  1 , Jashbhai B Prajapati  2 , Sreeja V Mudgal  3 , Himanshu V Pandya  4 , Uday Shankar Singh  5 , Sunil S Trivedi  6 , Ajay G Phatak  7 , Rupal M Patel  8
Affiliations

Effect of Probiotic Dietary Intervention on Calcium and Haematological Parameters in Geriatrics

Manisha Kalpesh Gohel et al. J Clin Diagn Res. 2016 Apr.

Abstract

Introduction: Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Sufficient calcium intake has been reported to support bone growth and prevent bone loss during the ageing process.

Aim: To determine the effect of Lactobacillus helveticus MTCC 5463 probiotic dietary intervention on serum calcium & haematological parameters in geriatric population.

Materials and methods: Healthy volunteers with age ranging from 64-74 years were recruited from the nearby residential areas in and around Anand, Gujarat. Study duration was from 2012 to 2015. Of the 112 subjects initially enrolled in the trial, 36 withdrew before the intervention because of not matching with criterias. Of the 76 participants, 5 subjects (4%) under test group and 12 subjects (11%) under placebo left the study. We had 59 subjects who successfully completed a double blind cross over trial. Probiotic fermented milk products (in form of "Lassi") was prepared by supplementing toned milk with honey and fermenting with probiotic Lactobacillus helveticus MTCC 5463 and Streptococcus thermophilus MTCC 5460. The final product had at least 10(8) CFU/ml of viable Lactobacillus helveticus MTCC 5463 at the time of feeding. During feeding period, 200 ml of fermented product containing the test strain to one group and a similar product but without the test strain as placebo were fed regularly at the time of breakfast in morning for 4 weeks. Subjects of each group were given a washout period of 4 weeks before they were crossed over and included to the other group. The study was approved by institutional ethics committee.

Results: The socio-demographic and clinical profiles were similar at baseline. The mean (SD) calcium level improved significantly in test {9.36 (0.45) vs 8.45 (0.61), p<0.001}. No significant effect was observed with respect to haemoglobin & haematological parameters.

Conclusion: The well-documented probiotic Lactobacillus helveticus MTCC 5463 confirmed increase in serum calcium level but no effect on haematological parameters when administered to geriatrics.

Keywords: Feeding trials; Healthy volunteers; Lactobacillus helveticus MTCC 5463; Lassi.

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Figures

[Table/Fig-1]:
[Table/Fig-1]:
Enrolment and randomization of participants.
[Table/Fig-2]:
[Table/Fig-2]:
Study design and sample collection.
See this image and copyright information in PMC

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