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. 2016 Mar;467(1):157-61.
doi: 10.1134/S1607672916020216. Epub 2016 May 20.

Expression and purification of an engineered human endothelin receptor B in a monomeric form

A V Mishin  1 , A P Luginina  2 , A P Potapenko  2 , V I Borshchevskiy  2   3 , V Katritch  4 , E Edelweiss  5   6 , I S Okhrimenko  2 , V I Gordeliy  2   6   7   8   3 , V G Cherezov  2   4
Affiliations

Expression and purification of an engineered human endothelin receptor B in a monomeric form

A V Mishin et al. Dokl Biochem Biophys. 2016 Mar.

Abstract

In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.

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