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. 2016 Jan-Apr;20(1):29-35.
doi: 10.4103/0973-029X.180922.

Immunohistochemical expression of p53 and its clinicopathological correlation with modified Anneroth's histological grading system

Kajal V Dave  1 , Monali Chalishazar  2 , Vishal R Dave  3 , Pritam Panja  4 , Manisha Singh  5 , Tapan G Modi  1
Affiliations

Immunohistochemical expression of p53 and its clinicopathological correlation with modified Anneroth's histological grading system

Kajal V Dave et al. J Oral Maxillofac Pathol. 2016 Jan-Apr.

Abstract

Introduction and objectives: Oral squamous cell carcinoma (OSCC) is an epithelial neoplasm generally beginning as focal overgrowth of altered stem cells near the basement membrane, moving upward and laterally, replacing the normal epithelium. Histopathological grading has been used for many decades in an attempt to predict the clinical behavior of oral squamous cell carcinoma. In the present study, Forty biopsies were studied for histological grading and p53 expression. The p53 expression was studied in relation to clinical parameters such as age, sex of patient and site of tumors. Relation between histological grade of malignancy and p53 protein expression was analysed. All cases were classified according to Anneroth's histological malignancy grading system (1987).

Materials and methods: 40 cases of OSCC were assessed for clinical parameters, Anneroth's histological grading and immunohistochemically stained with p53 protien.

Statistical analysis: The results obtained were analyzed using Spearman's Co-relation.

Observations and results: The positive expression of p53 was found in 62% of carcinomas studied. Positivity of p53 showed correlation with histological grade of malignancy and with individual parameters like degree of keratinization, nuclear polymorphism, number of mitoses and lymphoplasmacytic infiltration while showed a negative correlation with pattern of invasion.

Conclusion: Our study showed a significant correlation between parameters of tumor cell population, lymphoplasmacytic infiltration and p53 expression. A significant association between high grade of malignancy and p53 overexpression and insignificant correlation of p53 with age, sex of the patient and site of the tumor was found.

Keywords: Malignancy grading system; oral cancer; p53.

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Figures

Figure 1
Figure 1
Photomicrograph showing cellular dissociation in Grade III oral squamous cell carcinoma (H&E stain, ×100)
Figure 2
Figure 2
Photomicrograph showing intense and extensive p53 staining in Grade III tumors (IHC stain, ×100)
Figure 3
Figure 3
Photomicrograph showing keratin pearl formation in Grade I oral squamous cell carcinoma (H&E stain, ×100)
Figure 4
Figure 4
Photomicrograph showing P53 positivity to peripheral undifferentiated cells, whereas central keratinized cells are spared (IHC stain, ×400)
Figure 5
Figure 5
Photomicrograph showing abnormal mitotic figure (H&E stain, ×100)
Figure 6
Figure 6
Photomicrograph showing tumor invasion in small solid cords and strands (H&E stain, ×100)
Graph 1
Graph 1
Correlation of individual parameter of modified Anneroth's histological malignancy grading system with p53 positive expression. DK: Degree of Keratinization, NP: Nuclear polymorphism, NM: Number of mitoses, PI: Pattern of invasion, LPI: Lymphoplasmacytic infiltration
See this image and copyright information in PMC

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