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. 2016 Jan-Apr;20(1):59-65.
doi: 10.4103/0973-029X.180931.

Role of preprocedural rinse and high volume evacuator in reducing bacterial contamination in bioaerosols

T V Narayana  1 , Leeky Mohanty  2 , G Sreenath  3 , Pavani Vidhyadhari  3
Affiliations

Role of preprocedural rinse and high volume evacuator in reducing bacterial contamination in bioaerosols

T V Narayana et al. J Oral Maxillofac Pathol. 2016 Jan-Apr.

Abstract

Context: Microbial contamination, which occurs during dental procedures, has been a potential threat to dental professionals and individuals. There has been a growing concern over the role of bioaerosols in spread of various airborne infections and also to reduce the risk of bioaerosol contamination.

Aims: This study was to analyze the number of colony forming units (CFUs) in bioaerosols generated during ultrasonic scaling procedure as well as to evaluate the efficacy of chlorhexidine 0.12% (CHX) preprocedural mouth rinse and high volume evacuator (HVE) in minimizing the bioaerosol contamination.

Methods: About 45 individuals were divided into three Groups A, B and C. These groups underwent ultrasonic scaling before and after the use of CHX (0.12%), HVE and combination of CHX (0.12%) and HVE. Bioaerosols were collected on blood agar plates which were incubated at 37°C for 48 h, and the CFUs were counted with manual colony counting device. A comparison was also done between A versus B, B versus C and A versus C groups.

Statistical analysis used: Student's t-test.

Results: We found a significant reduction in the CFUs when CHX (0.12%) preprocedural rinse (P < 0), or HVE (P < 0.001) or combination of both CHX (0.12%) and HVE were employed (P < 0.001). Maximum reduction in CFUs was observed when CHX (0.12%) and HVE were used in combination as compared to their individual use. A moderate significance was seen between A versus C groups but not with B versus C groups and A versus B groups.

Conclusion: From our study, we conclude that individual methods such as CHX (0.12%) and HVE were useful to reduce the dental bioaerosols; however, combination of both CHX (0.12%) and HVE is more efficient to reduce dental bioaerosols than individual method.

Keywords: Bioaerosols; chlorhexidine; colony forming units; high volume evacuator.

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Figures

Figure 1
Figure 1
Position of the blood agar plate in the fiberglass box during the procedure
Figure 2
Figure 2
Position of the high volume evacuator during procedure
Figure 3
Figure 3
Colony forming units without the use of chlorhexidine (0.12%) preprocedural rinse
Figure 4
Figure 4
Reduction of colony forming units after the use of chlorhexidine (0.12%) preprocedural rinse
Figure 5
Figure 5
Colony forming units without the use of high volume evacuator
Figure 6
Figure 6
Reduction of colony forming units after the use of high volume evacuator
Figure 7
Figure 7
Colony forming units without the use of both chlorhexidine (0.12%) preprocedural rinse and high volume evacuator
Figure 8
Figure 8
Significant reduction of colony forming units with the use of chlorhexidine (0.12%) preprocedural rinse and high volume evacuator
Graph 1
Graph 1
Log Colony Forming Units with and without use of CHX
Graph 2
Graph 2
Log Colony Forming Units with and without use of HVE
Graph 3
Graph 3
Log CFU with and without use of both CHX and HVE
See this image and copyright information in PMC

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