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. 2016 Apr;39(2):143-5.

Molecular analysis for bacterial contamination in dental unit water lines

Akari Watanabe  1 , Naofumi Tamaki  2 , Miwa Matsuyama  1 , Susumu Kokeguchi  3
Affiliations
  • PMID: 27196554
Free article

Molecular analysis for bacterial contamination in dental unit water lines

Akari Watanabe et al. New Microbiol. 2016 Apr.
Free article

Abstract

Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.

Keywords: Bacterial contamination; Dental unit water line; Molecular analysis; PCR-DGGE.

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