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Review
. 2016 May 16;21(5):645.
doi: 10.3390/molecules21050645.

Finding and Producing Probiotic Glycosylases for the Biocatalysis of Ginsenosides: A Mini Review

Affiliations
Review

Finding and Producing Probiotic Glycosylases for the Biocatalysis of Ginsenosides: A Mini Review

Seockmo Ku. Molecules. .

Abstract

Various microorganisms have been widely applied in nutraceutical industries for the processing of phytochemical conversion. Specifically, in the Asian food industry and academia, notable attention is paid to the biocatalytic process of ginsenosides (ginseng saponins) using probiotic bacteria that produce high levels of glycosyl-hydrolases. Multiple groups have conducted experiments in order to determine the best conditions to produce more active and stable enzymes, which can be applicable to produce diverse types of ginsenosides for commercial applications. In this sense, there are various reviews that cover the biofunctional effects of multiple types of ginsenosides and the pathways of ginsenoside deglycosylation. However, little work has been published on the production methods of probiotic enzymes, which is a critical component of ginsenoside processing. This review aims to investigate current preparation methods, results on the discovery of new glycosylases, the application potential of probiotic enzymes and their use for biocatalysis of ginsenosides in the nutraceutical industry.

Keywords: biocatalysis; enzyme conversion; enzyme preparation; ginsenoside; probiotics; whole cell conversion.

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Figure 1
Figure 1
Biocatalysis of ginsenoside glycosides using microbial glycosyl hydrolases.
Figure 2
Figure 2
Application of probiotic enzymes prepared by multiple methods for the biotransformation of ginsenoside glycosides. The TLC profile was adapted and modified from Ku et al. [67].

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