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Comment
. 2016 May;24(5):851-4.
doi: 10.1038/mt.2016.76.

Endogenous Transposase Source in Human Cells Mobilizes piggyBac Transposons

Zoltán Ivics  1
Affiliations
Comment

Endogenous Transposase Source in Human Cells Mobilizes piggyBac Transposons

Zoltán Ivics. Mol Ther. 2016 May.
No abstract available

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Figures

Figure 1
Figure 1
piggyBac transposition and PGBD5. (a) Transposon-based nonviral gene delivery. A gene of interest (GOI, yellow box) flanked by inverted terminal repeat (ITR) sequences (black arrows) of the transposon vector is delivered into cells as a plasmid. This transposon can be mobilized from the plasmid vector into the host cell's genome if a transposase source is conditionally provided in cells. The transposase (brown spheres) binds to the ITRs, excises the gene construct from the donor plasmid, and integrates it at a chromosomal site. Because the transposase is only transiently present in cells, the genomically integrated transposon cannot move again. (b) Possible chromosomal mobilization of piggyBac (PB) transposons by PGBD5. If PGBD5 (purple spheres) is endogenously present in a cell that contains a PB transposon insertion, it may bind to the transposon ITRs and catalyze the excision and/or reinsertion of the transposon into other chromosomes. (c) Relative levels of PGBD5 expression in selected human primary cells. Data were obtained from the BioGPS website (http://biogps.org) by analyzing PGBD5 gene expression patterns in the cell types listed in the BioGPS Primary Cell Atlas, generated by a meta-analysis of publicly available microarray data sets derived from human primary cells. ESC, embryonic stem cell; HUVEC, human umbilical vein endothelial cell; iPSC, induced pluripotent stem cell; mRNA, messenger RNA; PGBD5, piggyBac-derived 5.
See this image and copyright information in PMC

Comment on

  • Genomic DNA transposition induced by human PGBD5.
    Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A. Henssen AG, et al. Elife. 2015 Sep 25;4:e10565. doi: 10.7554/eLife.10565. Elife. 2015. PMID: 26406119 Free PMC article.

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