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Comment
. 2016 May 19;62(4):477-8.
doi: 10.1016/j.molcel.2016.05.006.

CRISPR-Mediated Base Editing without DNA Double-Strand Breaks

Brian S Plosky  1
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Free article
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CRISPR-Mediated Base Editing without DNA Double-Strand Breaks

Brian S Plosky. Mol Cell. .
Free article

Abstract

Targeting point mutations using CRISPR/Cas9 so far has required efficient homologous recombination (HR) and donor oligonucleotides. In a recent Nature paper, Komor and colleagues (2016) describe a way to make specific base changes that does not depend on HR or donor DNA and does not involve making double-strand breaks.

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