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. 2016 Jul 22;60(8):5044-6.
doi: 10.1128/AAC.00470-16. Print 2016 Aug.

Frequency of BKC-1-Producing Klebsiella Species Isolates

Willames M B S Martins  1 , Adriana G Nicoletti  2 , Silvia R Santos  3 , Jorge L M Sampaio  4 , Ana C Gales  2
Affiliations

Frequency of BKC-1-Producing Klebsiella Species Isolates

Willames M B S Martins et al. Antimicrob Agents Chemother. .

Abstract

BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. The principal objective of this study was to evaluate the frequency of blaBKC-1 by testing a collection of Klebsiella isolates. Only 2 of 635 Klebsiella isolates (0.3%) carried blaBKC-1 The two BKC-1-producing isolates belonged to clonal complex 442 and possessed identical pulsed-field gel electrophoresis patterns. The blaBKC-1 gene was inserted into a 10-kb plasmid that was identical to the previously reported plasmid, p60136. The BKC-producing K. pneumoniae isolates presented also possessed other mechanisms for beta-lactam resistance, such as genes encoding extended-spectrum beta-lactamases and mutations in the genes ompK35 and ompK36, encoding the major porins.

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Figures

FIG 1
FIG 1
(A) Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the blaBKC-1 gene. PA, Pará; MA, Maranhão; CE, Ceará; RN, Rio Grande do Norte; BA, Bahia; MG, Minas Gerais; GO, Goiás; SP, São Paulo; RJ, Rio de Janeiro; PR, Paraná; SC, Santa Catarina; RS, Rio Grande do Sul. (B) Dendrogram and computer-generated image of PFGE results, analyzed by BioNumerics, for the BKC-1-producing K. pneumoniae isolates.
See this image and copyright information in PMC

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