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. 2016 Aug;85(4):416-8.
doi: 10.1016/j.diagmicrobio.2016.04.023. Epub 2016 May 5.

Implementation of Salmonella serotype determination using pulsed-field gel electrophoresis in a state public health laboratory

Dianna J Bopp  1 , Deborah J Baker  1 , Lisa Thompson  1 , Amy Saylors  1 , Timothy P Root  1 , Leeanna Armstrong  1 , Kara Mitchell  1 , Nellie B Dumas  1 , Kimberlee Arruda Musser  2
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Implementation of Salmonella serotype determination using pulsed-field gel electrophoresis in a state public health laboratory

Dianna J Bopp et al. Diagn Microbiol Infect Dis. 2016 Aug.

Abstract

We examined the use of pulsed-field gel electrophoresis (PFGE) to predict serotype for Salmonella isolates. Between 2012 and 2014 we assessed 4481 isolates, resulting in >90% assigned serotypes. PFGE is efficient for determining serotype in the majority of cases and results in expedited serotype determination, as well as cost savings.

Keywords: Cost savings; PFGE; Salmonella; Serotyping.

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