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. 2016 Mar-Apr;6(2):142-8.

Anti-proliferative and apoptotic effects of Ziziphus Jujube on cervical and breast cancer cells

Mohammad Reza Abedini  1 , Nafiseh Erfanian  2 , Habibollah Nazem  2 , Sara Jamali  3 , Reyhane Hoshyar  4
Affiliations

Anti-proliferative and apoptotic effects of Ziziphus Jujube on cervical and breast cancer cells

Mohammad Reza Abedini et al. Avicenna J Phytomed. 2016 Mar-Apr.

Abstract

Objective: Ziziphus Jujube (Jujube) plant has exhibited numerous medicinal and pharmacological properties including antioxidant and anti-inflammatory effects. This study was carried out to investigate its anti-cancer and pro-apoptotic abilities in human cervical and breast cancer cells in vitro.

Materials and methods: The cervical OV2008 and breast MCF-7 cancer cells were incubated with different concentrations of Jujube aqueous extraction (0-3 mg/ml) for various times (0-72 h). Cell viability was assessed by Trypan Blue and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of two apoptosis-related genes in treated cells evaluated by quantitative Real Time -PCR analysis.

Results: Jujube significantly inhibited cancer cell viability in a dose- and time- dependent manner. Herb-induced apoptosis was associated with enhanced expression of Bax and decreased Bcl2 gene leading eventually to a time-dependent six fold increase in the Bax/Bcl-2 ratio.

Conclusion: These results indicated that Jujube may be a natural potential and promising agent to prevent or treat human cancers.

Keywords: Apoptosis; Breast cancer; Cervical cancer; Ziziphus Jujube.

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Figures

Figure 1
Figure 1
Effect of Jujubeon MCS-7 cell viability. The cells were treated with different concentrations of Jujube for 0-72 hours. Data are expressed as mean ± SEM (n=3). Values are statistically significant at ***p<0.001, *p<0.05 vs. respective control group (One-way ANOVA followed by Tukey’s post hoc test).
Figure 2
Figure 2
Effect of Jujubeon MCF-10A cell viability. The cells treated with different concentrations of Jujube for 0-72 hours. Results are reported as the mean ± SEM (n=3; p>0.05). Values are not statistically significant compared to the control group (One-way ANOVA followed by Tukey’s post hoc test
Figure 3
Figure 3
OV2008 cells treated with 1.2 mg/ml Jujube for 0-36h. Jujube increased the gene expression of Bax and decreased Bcl2 expression in cells. Data represents relative gene expression (Target/β-actin) mean ± SEM of three experiments (n=3). Values are statistically significant at ***p<0.001, *p <0.05 vs. respective control group (One-way ANOVA followed by Tukey’s post hoc test
See this image and copyright information in PMC

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