Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy

doi:10.1007/978-3-319-32805-8_4

Review

. 2016:917:55-76.

doi: 10.1007/978-3-319-32805-8_4.

Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy

Tobias Unkauf¹, Sebastian Miethe¹, Viola Fühner¹, Thomas Schirrmann^{1

2}, André Frenzel^{1

2}, Michael Hust³

Affiliations

¹ Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
² YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
³ Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany. m.hust@tu-bs.de.

PMID: 27236552
PMCID: PMC7121732
DOI: 10.1007/978-3-319-32805-8_4

Review

Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy

Tobias Unkauf et al. Adv Exp Med Biol. 2016.

. 2016:917:55-76.

doi: 10.1007/978-3-319-32805-8_4.

Authors

Tobias Unkauf¹, Sebastian Miethe¹, Viola Fühner¹, Thomas Schirrmann^{1

2}, André Frenzel^{1

2}, Michael Hust³

Affiliations

¹ Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
² YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
³ Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany. m.hust@tu-bs.de.

PMID: 27236552
PMCID: PMC7121732
DOI: 10.1007/978-3-319-32805-8_4

Abstract

Antibody phage display is an in vitro technology to generate recombinant antibodies. In particular for pathogens like viruses or toxins, antibody phage display is an alternative to hybridoma technology, since it circumvents the limitations of the immune system. Phage display allows the generation of human antibodies from naive antibody gene libraries when either immunized patients are not available or immunization is not ethically feasible. This technology also allows the construction of immune libraries to select in vivo affinity matured antibodies if immunized patients or animals are available.In this review, we describe the generation of human and human-like antibodies from naive antibody gene libraries and antibodies from immune antibody gene libraries. Furthermore, we give an overview about phage display derived recombinant antibodies against viruses and toxins for diagnostics and therapy.

Keywords: Antibody; Antibody engineering; Pathogen; Phage display; Therapy; Toxins; Viruses; scFv.

PubMed Disclaimer

Cited by

Monoclonal Antibodies and Antibody Like Fragments Derived from Immunised Phage Display Libraries.
Ubah O, Palliyil S. Ubah O, et al. Adv Exp Med Biol. 2017;1053:99-117. doi: 10.1007/978-3-319-72077-7_6. Adv Exp Med Biol. 2017. PMID: 29549637 Free PMC article. Review.
Antibody Phage Display Technology for Sensor-Based Virus Detection: Current Status and Future Prospects.
Guliy OI, Evstigneeva SS, Khanadeev VA, Dykman LA. Guliy OI, et al. Biosensors (Basel). 2023 Jun 9;13(6):640. doi: 10.3390/bios13060640. Biosensors (Basel). 2023. PMID: 37367005 Free PMC article. Review.
Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.
Wang Y, Zhang C, Wang J, Knopp D. Wang Y, et al. Toxins (Basel). 2022 Jan 20;14(2):73. doi: 10.3390/toxins14020073. Toxins (Basel). 2022. PMID: 35202100 Free PMC article. Review.
Construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro.
Zhang F, Chen Y, Yang L, Zhu J. Zhang F, et al. Arch Virol. 2019 Apr;164(4):983-994. doi: 10.1007/s00705-019-04156-6. Epub 2019 Feb 7. Arch Virol. 2019. PMID: 30729994 Free PMC article.
In Vitro Characterization of Neutralizing Hen Antibodies to Coxsackievirus A16.
Mwale PF, Lee CH, Huang PN, Tseng SN, Shih SR, Huang HY, Leu SJ, Huang YJ, Chiang LC, Mao YC, Wang WC, Yang YY. Mwale PF, et al. Int J Mol Sci. 2021 Apr 16;22(8):4146. doi: 10.3390/ijms22084146. Int J Mol Sci. 2021. PMID: 33923724 Free PMC article.

See all "Cited by" articles

References

1. Adams GP, Weiner LM. Monoclonal antibody therapy of cancer. Nat Biotechnol. 2005;23:1147–1157. doi: 10.1038/nbt1137. - DOI - PubMed
1. Alonso-Ruiz A, Pijoan JI, Ansuategui E, et al. Tumor necrosis factor alpha drugs in rheumatoid arthritis: systematic review and metaanalysis of efficacy and safety. BMC Musculoskelet Disord. 2008;9:52. doi: 10.1186/1471-2474-9-52. - DOI - PMC - PubMed
1. Amersdorfer P, Wong C, Chen S, et al. Molecular characterization of murine humoral immune response to botulinum neurotoxin type A binding domain as assessed by using phage antibody libraries. Infect Immun. 1997;65:3743–3752. - PMC - PubMed
1. Amersdorfer P, Wong C, Smith T, et al. Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries. Vaccine. 2002;20:1640–1648. doi: 10.1016/S0264-410X(01)00482-0. - DOI - PubMed
1. Amaro I, Riaño-Umbarila L, Becerril B, Possani LD. Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion. Immunol Lett. 2011;139:73–79. doi: 10.1016/j.imlet.2011.05.002. - DOI - PubMed

Publication types

Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

[1] Adams GP, Weiner LM. Monoclonal antibody therapy of cancer. Nat Biotechnol. 2005;23:1147–1157. doi: 10.1038/nbt1137. - DOI - PubMed

[2] Adams GP, Weiner LM. Monoclonal antibody therapy of cancer. Nat Biotechnol. 2005;23:1147–1157. doi: 10.1038/nbt1137. - DOI - PubMed

[3] Alonso-Ruiz A, Pijoan JI, Ansuategui E, et al. Tumor necrosis factor alpha drugs in rheumatoid arthritis: systematic review and metaanalysis of efficacy and safety. BMC Musculoskelet Disord. 2008;9:52. doi: 10.1186/1471-2474-9-52. - DOI - PMC - PubMed

[4] Alonso-Ruiz A, Pijoan JI, Ansuategui E, et al. Tumor necrosis factor alpha drugs in rheumatoid arthritis: systematic review and metaanalysis of efficacy and safety. BMC Musculoskelet Disord. 2008;9:52. doi: 10.1186/1471-2474-9-52. - DOI - PMC - PubMed

[5] Amersdorfer P, Wong C, Chen S, et al. Molecular characterization of murine humoral immune response to botulinum neurotoxin type A binding domain as assessed by using phage antibody libraries. Infect Immun. 1997;65:3743–3752. - PMC - PubMed

[6] Amersdorfer P, Wong C, Chen S, et al. Molecular characterization of murine humoral immune response to botulinum neurotoxin type A binding domain as assessed by using phage antibody libraries. Infect Immun. 1997;65:3743–3752. - PMC - PubMed

[7] Amersdorfer P, Wong C, Smith T, et al. Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries. Vaccine. 2002;20:1640–1648. doi: 10.1016/S0264-410X(01)00482-0. - DOI - PubMed

[8] Amersdorfer P, Wong C, Smith T, et al. Genetic and immunological comparison of anti-botulinum type A antibodies from immune and non-immune human phage libraries. Vaccine. 2002;20:1640–1648. doi: 10.1016/S0264-410X(01)00482-0. - DOI - PubMed

[9] Amaro I, Riaño-Umbarila L, Becerril B, Possani LD. Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion. Immunol Lett. 2011;139:73–79. doi: 10.1016/j.imlet.2011.05.002. - DOI - PubMed

[10] Amaro I, Riaño-Umbarila L, Becerril B, Possani LD. Isolation and characterization of a human antibody fragment specific for Ts1 toxin from Tityus serrulatus scorpion. Immunol Lett. 2011;139:73–79. doi: 10.1016/j.imlet.2011.05.002. - DOI - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy

Affiliations

Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources