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. 2016:2016:6409546.
doi: 10.1155/2016/6409546. Epub 2016 Apr 28.

A Comparative Evaluation of the Mechanical Properties of Two Calcium Phosphate/Collagen Composite Materials and Their Osteogenic Effects on Adipose-Derived Stem Cells

Qing Li  1 , Tong Wang  2 , Gui-Feng Zhang  3 , Xin Yu  4 , Jing Zhang  4 , Gang Zhou  4 , Zhi-Hui Tang  5
Affiliations

A Comparative Evaluation of the Mechanical Properties of Two Calcium Phosphate/Collagen Composite Materials and Their Osteogenic Effects on Adipose-Derived Stem Cells

Qing Li et al. Stem Cells Int. 2016.

Abstract

Adipose-derived stem cells (ADSCs) are ideal seed cells for use in bone tissue engineering and they have many advantages over other stem cells. In this study, two kinds of calcium phosphate/collagen composite scaffolds were prepared and their effects on the proliferation and osteogenic differentiation of ADSCs were investigated. The hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite scaffolds (HTPSs), which have an additional β-tricalcium phosphate, resulted in better proliferation of ADSCs and showed osteogenesis-promoting effects. Therefore, such composite scaffolds, in combination with ADSCs or on their own, would be promising for use in bone regeneration and potential clinical therapy for bone defects.

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Figures

Figure 1
Figure 1
Pictures and SEM images of HTPS and HAPS. (a) HTPS group; (b) HAPS group; (c) SEM images of the HTPS group, 500x; and (d) SEM images of the HAPS group, 500x.
Figure 2
Figure 2
FTIR patterns of HTPS and HAPS. Both the groups share the same organic and inorganic composition. Note: green line: HTPS; purple line: HAPS. Dashed lines indicate similar symbolic peaks of the two groups: 1030 cm−1: v 3 vibration of PO4 3−; 602, 566 cm−1: v 4 vibration of PO4 3−; 1547 cm−1: Amide II; and 1600 cm−1: Amide I.
Figure 3
Figure 3
XRD spectra of HTPS and HAPS. Note: green line: HTPS; purple line: HAPS. Note: round symbols indicate the symbolic peaks of hydroxyapatite and triangles at 23.4°–23.7° and 26.9°–27.4° demonstrate the symbolic peaks of β-TCP in CHTS.
Figure 4
Figure 4
Young's moduli of HTPS and HAPS.
Figure 5
Figure 5
Porosity analysis of HTPS and HAPS.
Figure 6
Figure 6
Water absorption rate of HTPS and HAPS.
Figure 7
Figure 7
MTT line graph of HTPS and HAPS.
Figure 8
Figure 8
Gene expression of ALP, BMP-2, OCN, OPN, COL1A1, and COL2A in ADSCs cultured on HAPS and HTPS for 3 and 5 days. Note: ∗ P < 0.05, ∗∗ P < 0.01.
Figure 9
Figure 9
Fluorescence images of the ADSC seeded on HAPS and HTPS. Note: (a and b): ADSCs on HAPS at 24 h, 10x and 20x objective lenses, respectively, and (c and d): ADSCs on HTPS at 24 h, 10x and 20x objective lenses, respectively.
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