[PHF10 isoforms are phosphorylated in the PBAF mammalian chromatin remodeling complex]

Abstract

Chromatin remodeling complex PBAF(SWI/SNF) alters the structure of chromatin and controls gene expression. PHF10 is a specific subunit of PBAF complex and is expressed as four isoforms in mammalian cells. We demonstrated that all isoforms are expressed in various human cell types of different histological origins. All four isoforms are extensively phosphorylated and their phosphorylation level is depended on the cell type. Phosphorylation of PHF10 isoforms occurs while they are incorporated as a subunit of the PBAF complex, and therefore phosphorylation of PHF10 isoforms may play an essential role in regulation of PBAF complex's function and mechanism of action.

Keywords: PHF10; SWI/SNF; chromatin remodeling; chromatin-remodeling complex; gene expression; posttranslational modifications; protein isoforms; protein phosphorylation.