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. 2016 Jun 1:6:26985.
doi: 10.1038/srep26985.

Microbiological profiles of sputum and gastric juice aspirates in Cystic Fibrosis patients

H Al-Momani  1 , A Perry  2 , C J Stewart  3 , R Jones  1 , A Krishnan  1 , A G Robertson  1 , S Bourke  4 , S Doe  4 , S P Cummings  5 , A Anderson  4 , T Forrest  4 , S M Griffin  4 , M Brodlie  1 , J Pearson  1 , C Ward  1
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Microbiological profiles of sputum and gastric juice aspirates in Cystic Fibrosis patients

H Al-Momani et al. Sci Rep. .

Abstract

Gastro-Oesophageal Reflux (GOR) is a key problem in Cystic Fibrosis (CF), but the relationship between lung and gastric microbiomes is not well understood. We hypothesised that CF gastric and lung microbiomes are related. Gastric and sputum cultures were obtained from fifteen CF patients receiving percutaneous endoscopic gastrostomy feeding. Non-CF gastric juice data was obtained through endoscopy from 14 patients without lung disease. Bacterial and fungal isolates were identified by culture. Molecular bacterial profiling used next generation sequencing (NGS) of the 16S rRNA gene. Cultures grew bacteria and/or fungi in all CF gastric juice and sputa and in 9/14 non-CF gastric juices. Pseudomonas aeruginosa(Pa) was present in CF sputum in 11 patients, 4 had identical Pa strains in the stomach. NGS data from non-CF gastric juice samples were significantly more diverse compared to CF samples. NGS showed CF gastric juice had markedly lower abundance of normal gut bacteria; Bacteroides and Faecalibacterium, but increased Pseudomonas compared with non-CF. Multivariate partial least squares discriminant analysis demonstrated similar bacterial profiles of CF sputum and gastric juice samples, which were distinct from non-CF gastric juice. We provide novel evidence suggesting the existence of an aerodigestive microbiome in CF, which may have clinical relevance.

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Figures

Figure 1
Figure 1
(A) Bar plot showing the relative abundance of each operational taxonomic unit (OTU) within non-CF gastric juice (GJ), CF gastric juice (CFGJ), and sputum samples (CFS). (B) Shannon Diversity Index of CF samples (CFGJ and CFS) and non-CF GJ.
Figure 2
Figure 2. Partial least square discriminant analysis (PLS-DA) score scatter plot of all samples.
Non-CF gastric juice – GJ; CF gastric juice – CFGJ; Sputum samples – CFS. The Axes represent a % of variance. The CF gastric juice and sputum samples show a close relationship. In contrast the Gastric Juice samples from control patients are widely variable and clearly distinct from the CF sputum and gastric juice clustering.
See this image and copyright information in PMC

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