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. 2016 Jan-Feb;6(1):67-76.

Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta

Abbasali Abbasnezhad  1 , Saeed Niazmand  2 , Maryam Mahmoudabady  3 , Mohammad Soukhtanloo  4 , Seyed Abdolrahim Rezaee  5 , Seyed Mojtaba Mousavi  1
Affiliations

Nigella sativa seed decreases endothelial dysfunction in streptozotocin-induced diabetic rat aorta

Abbasali Abbasnezhad et al. Avicenna J Phytomed. 2016 Jan-Feb.

Abstract

Objective: Diabetes is an important risk factor for cardiovascular events. The great percent of morbidity in patients with diabetes is due to endothelial dysfunction. The present study investigated the effects of hydroalcholic extract of Nigella sativa (N. sativa) on contractile and dilatation response of isolated aorta in streptozotocin (STZ)-induced diabetic rat.

Materials and methods: Rats were divided into six experimental groups (control, untreated STZ-diabetic, and N. sativa hydroalcholic extract or metformin-treated diabetic rats). Treated rats received N. sativa extract (100, 200, and 400 mg/kg) or metformin (300 mg/kg) by gavage, daily for 6 weeks. Isolated rat thoracic rings were mounted in an organ bath system then contractile and dilatation responses induced by phenylephrine (PE), acetylcholine (ACh), potassium chloride (KCl), and sodium nitroprusside (SNP) were evaluated in different situations.

Results: The lower concentrations of N. sativa seed extract (DE 100 and DE 200) and metformin significantly reduced the contractile responses to higher concentrations of PE (10(-6) - 10(-5) M) compared to diabetic group (p<0.05 to p<0.01). The relaxation response to Ach 10(-8) M, was increased in DE 200 and metformin groups compared to diabetic group (p<0.05). The relaxation responses to Ach 10(-7) - 10(-5) M were significantly higher in all treated groups compared to diabetic group (p<0.05 to p<0.001).

Conclusion: Chronic administration of N. sativa seed extract has a significant hypoglycemic effect and improves aortic reactivity to vasoconstrictor and vasodilator agents in STZ-induced diabetic rats.

Keywords: Diabetes mellitus; Endothelial dysfunction; Isolated aorta; Nigella sativa; Rat.

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Figures

Figure 1
Figure 1
The contractile response of aortic rings to cumulative concentrations of phenylepherine (10-9 - 10-5 M) in control (C), diabetic (D), N. sativa seed extract (DE 100, DE 200, and DE 400), and metformin (DM) treated groups. Values are presented as means±SEM (n = 8). * p<0.05, ** p<0.01 compared to diabetic group and + p<0.05 compared to control group
Figure 2
Figure 2
The contractile response of aortic rings to cumulative concentrations of KCL (20-60 mM) in control (C), diabetic (D), N. sativa seed extract (DE 100, DE 200, and DE 400), and metformin (DM) treated groups. Values are presented as means±SEM (n = 8). * p<0.05, *** p<0.001 compared to diabetic group and +p<0.05 compared to control group
Figure 3
Figure 3
The relaxation responses of aortic rings to cumulative concentrations of acetylcholine (10-8 - 10-5 M) in control (C), diabetic (D), N. sativa seed extract (DE 100, DE 200, and DE 400), and metformin (DM) treated groups. Values are presented as means±SEM (n=8). *p<0.001, *** p<0.001 compared to diabetic group and + p<0.05, +++ p<0.001 compared to control group
Figure 4
Figure 4
The relaxation responses of aortic rings to cumulative concentrations of sodium nitroprusside (10-9 - 10-6 M) in control (C), diabetic (D), N. sativa seed extract (DE 100, DE 200, and DE 400), and metformin (DM) treated groups. Values are presented as means±SEM (n = 8
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References

    1. Abebe W, Harris KH, Macleod KM. Enhanced contractile responses of arteries from diabetic rats to alpha 1-adrenoceptor stimulation in the absence and presence of extracellular calcium. J Cardiovasc Pharmacol. 1990;16:239–248. - PubMed
    1. Ahmad A, HusainA , Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F, Kishore K. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3:337–352. - PMC - PubMed
    1. Ahmad S, Beg ZH. Elucidation of mechanisms of actions of thymoquinone-enriched methanolic and volatile oil extracts from Nigella sativa against cardiovascular risk parameters in experimental hyperlipidemia. Lipids Health Dis. 2013;12:86. - PMC - PubMed
    1. Calvert JW, Gundewar S, Jha S, Greer JJ, Bestermann WH, Tian R, Lefer DJ. Acute metformin therapy confers cardioprotection against myocardial infarction via AMPK-eNOS-mediated signaling. Diabetes. 2008;57:696–705. - PubMed
    1. Capellini VK, Baldo CF, Celotto AC, Batalhao ME, Carnio EC, Rodrigues AJ, Evora PR. Oxidative stress is not associated with vascular dysfunction in a model of alloxan-induced diabetic rats. Arq Bras Endocrinol Metabol. 2010;54:530–539. - PubMed

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