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. 2016 Jan-Feb;6(1):86-94.

Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney

Reza Mohebbati  1 , Mohammad Naser Shafei  2 , Mohammad Soukhtanloo  3 , Noema Mohammadian Roshan  4 , Abolfazl Khajavi Rad  5 , Akbar Anaeigoudari  6 , Sara Hosseinian  1 , Sareh Karimi  1 , Farimah Beheshti  1
Affiliations

Adriamycin-induced oxidative stress is prevented by mixed hydro-alcoholic extract of Nigella sativa and Curcuma longa in rat kidney

Reza Mohebbati et al. Avicenna J Phytomed. 2016 Jan-Feb.

Abstract

Objective: Inflammation and oxidative stress is considered to have a crucial role in induction of nephropathy. Curcuma longa (C. longa) and Nigella sativa (N. sativa) have anti-inflammatory and antioxidant effects. This study was designed to investigate the effect of mixed hydro-alcoholic extract of N.sativa and C. longa on the oxidative stress induced by Adriamycin (ADR) in rat kidney.

Materials and methods: The animals were divided into 6 groups: control (CO), ADR, Adriamycin+ Vitamin C (ADR+VIT C), C. longa extract+ Adriamycin (C.LE+ADR), N. sativa extract+ Adriamycin (N.SE+ADR) and C. longa extract+ N. sativa extract + Adriamycin (N.S+C.L+ADR). ADR (5mg/kg) was injected intravenously, whereas VITC (100mg/kg) and extract of C. longa (1000mg/kg) and N. sativa (200mg/kg) were administrated orally. Finally, the renal tissue, urine and blood samples were collected and submitted to measure of redox markers, osmolarity and renal index.

Results: The renal content of total thiol and superoxide dismutase (SOD) activity significantly decreased and Malondialdehyde (MDA) concentration increased in Adriamycin group compared to control group. The renal content of total thiol and SOD activity significantly enhanced and MDA concentration reduced in treated-mixed extract of C. longa and N. sativa along with ADR group compared to ADR group. The mixed extract did not restore increased renal index percentage induced by ADR. There also was no significant difference in urine and serum osmolarity between the groups.

Conclusion: hydro-alcoholic extracts of N.sativa and C.longa led to an improvement in ADR-induced oxidative stress and mixed administration of the extracts enhanced the aforementioned therapeutic effect.

Keywords: Adriamycin; Curcuma longa; Nigella sativa; Oxidative stress.

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Figures

Figure 1
Figure 1
Comparison of the MDA concentrations in renal tissue of experimental groups. Data are presented as Mean ± SEM (n= 8 in each group). **p<0.01 compared to control group. #p<0.05, ##p<0.01 and ###p< 0.001 compared to ADR group. &p<0.05 compared to N.S E+ ADR group. @@p<0.01 compared to C.L E + ADR group. ^p<0.05 compared to VIT C + ADR group
Figure 2
Figure 2
Comparison of the total thiol concentration in renal tissue of experimental groups. Data are presented as Mean ± SEM (n= 8 in each group). **p<0.01 compared to control group. ##p<0.01 and ###p< 0.001 compared to ADR group. &p<0.05 compared to N.S E+ ADR group. ^p<0.05 compared to VIT C + ADR group.
Figure 3
Figure 3
Comparison of the SOD activity in renal tissue of six groups. Data are presented as Mean ± SEM (n= 8 in each group). **P<0.01 compared with control group. #P<0.05 and ###P< 0.001 compared with ADR group. &P<0.05 compared to N.S E+ ADR group. @@P<0.01 compared to C.L E + ADR group. ^P<0.05 compared to VIT C + ADR group
Figure 4
Figure 4
Comparison of renal index percentage in six groups. Data are presented as Mean ± SEM (n= 8 in each group). *p<0.05 compared with control group
Figure 5
Figure 5
Comparison of urine and serum osmolarity between the six groups in different days. Data are presented as Mean ± SEM (n= 8 in each group). There was no significant difference in urine and serum osmollarity between groups
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