Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Apr;45(4):485-93.

Characterization of Staphylococcus aureus Biofilm Formation in Urinary Tract Infection

Masoud Yousefi  1 , Mohammad Reza Pourmand  2 , Fatemeh Fallah  1 , Ali Hashemi  1 , Rahil Mashhadi  3 , Ali Nazari-Alam  1
Affiliations

Characterization of Staphylococcus aureus Biofilm Formation in Urinary Tract Infection

Masoud Yousefi et al. Iran J Public Health. 2016 Apr.

Abstract

Background: The aim of this study was to investigate the antibiotic susceptibility pattern as well as the phenotypic and genotypic biofilm formation ability of Staphylococcus aureus isolates from patients with urinary tract infection (UTI).

Methods: A total of 39 isolates of S. aureus were collected from patients with UTI. The antibiotic susceptibility patterns of the isolates were determined by the Kirby-Bauer disk-diffusion. We used the Modified Congo red agar (MCRA) and Microtiter plate methods to assess the ability of biofilm formation. All isolates were examined for determination of biofilm related genes, icaA, fnbA, clfA and bap using PCR method.

Results: Linezolid, quinupristin/dalfopristin and chloramphenicol were the most effective agents against S. aureus isolates. Overall, 69.2% of S. aureus isolates were biofilm producers. Resistance to four antibiotics such as nitrofurantoin (71.4% vs. 28.6%, P=0.001), tetracycline (57.7% vs. 42.3%, P=0.028), erythromycin and ciprofloxacin (56% vs. 44%, P=0.017) was higher among biofilm producers than non-biofilm producers. The icaA, fnbA and clfA genes were present in all S. aureus isolates. However, bap gene was not detected in any of the isolates.

Conclusion: Our findings reinforce the role of biofilm formation in resistance to antimicrobial agents. Trimethoprimsulfamethoxazole and doxycycline may be used as an effective treatment for UTI caused by biofilm producers S. aureus. Our results suggest that biofilm formation is not dependent to just icaA, fnbA, clfA and bap genes harbor in S. aureus strains.

Keywords: Antibiotic resistance; Biofilm formation; Staphylococcus aureus; Urinary tract infection.

PubMed Disclaimer

Figures

Fig. 1:
Fig. 1:
Antimicrobial resistance pattern of S. aureus isolates (MRSA and MSSA).
Fig. 2:
Fig. 2:
Colony morphologies of S. aureus isolates on the modified Congo red agar medium
Fig. 3:
Fig. 3:
Amplification of icaA, fnbA, clfA genes from S. aureus isolates. Lane M, DNA marker (100 bp); Lane1 and 2, clfA (657 bp); Lane 4 and 5, fnbA (191 bp); Lane 7 and 8, icaA (509 bp); Lane 3, 6 and 9, negative controls
See this image and copyright information in PMC

References

    1. Ranjbar R, Haghi-Ashtiani M, Jafari NJ, Abedini M. (2009). The prevalence and antimicrobial susceptibility of bacterial uropathogens isolated from pediatric patients. Iran J Public Health, 38 ( 2): 134–38.
    1. Mobaleghi J, Salimizand H, Beiranvand S, Membari Sh, Kalantar E. (2012). Extended spectrum beta-lactamases in urinary isolates of Escherichia coli in five Iranian hospitals. Int J Infect Dis, 5: 35–6.
    1. Taheri PA, Navabi B, Khatibi E. (2013). Frequency and susceptibility of bacteria caused urinary tract infection in neonates: eight-year study at neonatal division of bahrami children's hospital, Tehran Iran. Iran J Public Health, 42 ( 10): 1126–33. - PMC - PubMed
    1. Kayas L, Yolbas I, Ece A, Kayas Y, Kocamaz H. (2011). Causative agents and antibiotic susceptibilities in children with urinary tract infection. J Microbiol Infect Dis, 1 ( 101): 17–21.
    1. Tayebi Z, Seyedjavadi SS, Goudarzi M, Rahimi MK, Boromandi S, Bostanabad SZ. (2014). Frequency and antibiotic resistance pattern in gram positive uropathogenes isolated from hospitalized patients with urinary tract infection in Tehran, Iran. J Genes Microb Immun, 1 – 9 .

LinkOut - more resources