Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Apr;9(4):1754-8.
doi: 10.1128/mcb.9.4.1754-1758.1989.

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

T M Underhill  1 , W F Flintoff
Affiliations

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer

T M Underhill et al. Mol Cell Biol. 1989 Apr.

Abstract

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Biochem Pharmacol. 1987 May 15;36(10):1659-67 - PubMed
    1. Exp Cell Res. 1987 Apr;169(2):395-407 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5853-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6546-9 - PubMed
    1. Biochim Biophys Acta. 1988 Apr 22;939(3):533-41 - PubMed

Publication types

LinkOut - more resources