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. 1989 Mar;14(3):229-33.
doi: 10.1007/BF00971315.

Developmental changes in high-affinity uptake of GABA by cultured neurons

V J Balcar  1 , K L Hauser, H Demieville
Affiliations

Developmental changes in high-affinity uptake of GABA by cultured neurons

V J Balcar et al. Neurochem Res. 1989 Mar.

Abstract

High-affinity uptake of [3H]gamma-aminobutyric acid (GABA) was studied in cultures of neonatal rat cortical neurons grown on pre-formed monolayers of non-neuronal (glial) cells. Both the maximum rate (Vmax) and, to a smaller extent, the Km of [3H]GABA uptake increased with time. In addition, in parallel with these changes, 2,4-diaminobutyric acid and cis-3-aminocyclohexane-1-carboxylic acid (ACHC), compounds which are considered typical substrate/inhibitors of GABA uptake in neurons, became progressively stronger inhibitors of [3H]GABA uptake. Consequently, the present results may mean that the studies using uptake of [3H]GABA, [3H]ACHC, or [3H]DABA as a specific marker for GABAergic neurons differentiating during the ontogenetic development of the central nervous system may have to be interpreted with caution.

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References

    1. Brain Res. 1975 Jun 6;90(1):1-21 - PubMed
    1. J Cell Biol. 1973 Nov;59(2 Pt 1):329-45 - PubMed
    1. Brain Res. 1980 May 19;190(1):139-52 - PubMed
    1. J Neurochem. 1986 Mar;46(3):739-44 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2981-5 - PubMed