Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2016 Jul;32(7):106.
doi: 10.1007/s11274-016-2045-0. Epub 2016 Jun 4.

Agrobacterium tumefaciens-mediated transformation of Botryosphaeria dothidea

Liang Chen  1   2 , Qun Wang  3 , Hua Chen  4 , Gengwu Sun  1 , Huixiang Liu  5 , Hongkai Wang  6
Affiliations

Agrobacterium tumefaciens-mediated transformation of Botryosphaeria dothidea

Liang Chen et al. World J Microbiol Biotechnol. 2016 Jul.

Abstract

Botryosphaeria dothidea is a severe causal agent of die-back and cankers of many woody plants and causes great losses in many regions. The pathogenic mechanism of this pathogen has not been well explored due to lack of mutants and genetic information. In this study, we developed an Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for B. dothidea protoplasts using vector pBHt2 containing the hph gene as a selection marker under the control of trp C promoter. Using this protocol we successfully generated the B. dothidea transformants with efficiency about 23 transformants per 10(5) protoplasts. This is the first report of genetic transformation of B. dothidea via ATMT and this protocol provides an effective tool for B. dothidea genome manipulation, gene identification and functional analysis.

Keywords: ATMT; Botryosphaeria dothidea; Hygromycin B; T-DNA; hph.

PubMed Disclaimer

References

    1. Curr Genet. 2005 Jul;48(1):1-17 - PubMed
    1. Phytopathology. 1997 Sep;87(9):967-72 - PubMed
    1. Science. 1988 Apr 8;240(4849):204-7 - PubMed
    1. Nat Biotechnol. 1998 Sep;16(9):839-42 - PubMed
    1. EMBO J. 1995 Jul 3;14(13):3206-14 - PubMed

MeSH terms

LinkOut - more resources